131例强直性脊柱炎患者Treg细胞和淋巴细胞亚群分析.docVIP

131例强直性脊柱炎患者Treg细胞和淋巴细胞亚群分析 　　[摘要] 目的 ?^察强直性脊柱炎（AS）患者外周血Treg细胞与淋巴细胞各亚群的变化及HLA-B27的表达，探讨各自在AS患者发展中的作用。 方法 应用流式细胞学方法，选取2013年2月～2016年12月于无锡市第二人民医院骨科和风湿科就诊的AS患者131例为AS组，选取健康体检者127例为正常对照组，进行外周血Treg细胞、淋巴细胞亚群、HLA-B27的检测，分析Treg细胞、淋巴细胞亚群、HLA-B27与AS患者的关系。 结果 131例AS组中HLA-B27检测阳性为123例（93.89%），正常对照组中HLA-B27检测阳性为2例（1.57%），差异有统计学意义（P 0.05）。 结论 HLA-B27与AS有强相关性。外周血中淋巴细胞各亚群组分在AS与正常对照组中存在明显差异。同时外周血中Treg细胞在AS患者中明显降低。以上发现提示细胞免疫和体液免疫可能在AS中发挥重要作用。 　　[关键词] 强直性脊柱炎；Treg细胞；淋巴细胞亚群；HLA-B27 　　[中图分类号] R681.5 [文献标识码] A [文章编号] 1673-7210（2017）10（a）-0046-03 　　[Abstract] Objective To analyze the changes of regulatory T cells （Treg cells） and the subsets of lymphocytes as well as the HLA-B27 expression in peripheral blood of ankylosing spondylitis （AS） patients， and then explore their roles in the development of AS. Methods Flow cytometry was applied. From February 2013 to December 2016， Department of Orthopedic and Rheumatology in Wuxi Second Peoples Hospital， 131 cases of AS patients were selected as AS group， 127 healthy subjects were selected as normal control group. The peripheral blood Treg cells， lymphocyte subsets and HLA-B27 were detected. The relationship of Treg cells， lymphocyte subsets， HLA-B27 and AS patients were analyzed. Results 131 cases of AS group， HLA-B27 positive for 123 cases （93.89%）， the positive rate of HLA-B27 in normal control group was 2 cases （1.57%）， the difference was statistically significant （P 0.05）. Conclusion HLA-B27 has a strong correlation with AS. There are significant differences in the subgroups of lymphocytes in peripheral blood between AS and normal control group. At the same time， peripheral blood Treg cells in AS patients are significantly reduced. The above findings suggest that cellular immunity and humoral immunity may play an important role in AS. 　　[Key words] Ankylosing spondylitis； Regulatory T cells； Lymphocyte subsets； HLA-B27 　　强直性脊柱炎（ankylosing spondylitis，AS）是一种全身免疫疾病，以慢性炎症为主，它可侵犯脊柱关节，累及肌腱、滑膜等关节，大部分患者有骶髂关节炎。AS青壮年多发，有家族聚集性，可能与遗传相关。其发病机制