纤溶豆豉的制备及其对小鼠溶栓效果的评价-航空制造技术.PDF

228 2012, Vol. 33, No. 03 食品科学 ※营养卫生 李小东，王成涛 * ，赵 磊，杨雪莲，张佳婵 (北京工商大学 食品添加剂与配料北京高校工程研究中心，食品风味化学北京市重点实验室，北京 100048) ：利用动物血栓模型，饲喂纤溶豆豉来研究其在动物体内的溶栓作用。通过小鼠腰背部皮下注射 10mg/mL 角叉菜胶溶液诱发小鼠尾静脉形成血栓，实验组 A 饲喂豆豉纤溶豆豉并自由饮用纤溶豆豉提取液，对照组 B(模型 小鼠) 、对照组 C( 正常小鼠) 分别饲喂全价营养鼠粮，并自由饮水。结果表明：各组小鼠生长情况良好；饲喂纤 溶豆豉的实验组 A 小鼠体质量先降低后增高，饲喂 5d 以后尾部形成的血栓长度逐渐变短，10d 时凝血酶原时间、 凝血酶时间稍有延长，但差异不显著，15d 后凝血酶原时间、凝血酶时间均明显延长(P ＜0.05) ，血浆中纤维蛋白 原(FIB)含量相应降低，D - 二聚体在饲喂 10d 后呈阳性；而饲喂全价营养鼠粮的对照组 B 及注射生理盐水的对照组 C ，其PT 、TT 差异均不显著。因此食用纤溶豆豉能激活机体纤溶系统，抑制凝血系统作用，有助于预防和辅 助治疗血栓性疾病。 中国豆豉；小鼠血栓模型；血栓长度；纤溶活性；角叉菜胶 Preparation of Fibrinolytic Douchi and Evaluation of Its Thrombolytic Effectiveness in vivo LI Xiao-dong ，WANG Cheng-tao* ，ZHAO Lei ，YANG Xue-lian ，ZHANG Jia-chan (Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University, Beijing 100048, China) Abstract ：The effect of feeding Douchi was evaluated on mouse thrombosis induced by subcutaneous injection with 10 mg/mL carrageenan at the back waist of mice. ICR mice were divided into three groups: Douchi treatment group (thrombosis model mice fed Douchi and normal saline extract from Douchi), model control group (thrombosis model mice fed full nutrition diet and distilled water) and normal control group (normal mice fed full nutrition diet and distilled water). The results showed that the mice in the Douchi treatment group exhibited an initial decrease and subsequent increase in body weight, a slight increase in prothrombin time (PT) and thrombin time (TT), and a reduction in plasma fibrinogen (FIB) during the experiment. The detection of D-dimer was positive after 10 days of feeding and PT and TT were n