陆地棉体细胞胚胎发生过程中的细胞外基质研究-中国农业科学院棉花.PDFVIP

花 学 报 Cotton Science 20 12 24 渊2 冤院167~ 175 地棉体细胞胚胎发生过程中的细胞外基质研究 海红 袁有禄 刘传亮 张朝军 武芝霞 张雪妍 李付广* 中国农业科学院棉花研究所/ 棉花生物学国家重点实验室袁河南安阳455004冤 摘要院研究棉花体细胞胚胎发生对于促进棉花基因工程育种具有重要意义遥 利用扫描电镜和透射电镜对陆地 棉体细胞胚胎发生过程的研究发现袁 细胞外基质在棉花体细胞胚胎发生的不同阶段呈现出有规律的动态变 化遥 当普通愈伤组织细胞发育成胚性愈伤组织时袁细胞外基质就会出现曰随着原胚的形成袁细胞外基质就会形 成发达的网络状结构曰随着胚状体的进一步形成和发育袁细胞外基质则逐渐降解遥 在愈伤组织和非胚性愈伤组 织 袁始终未发现细胞外基质遥 结果表明袁细胞外基质与体细胞胚胎发生能力关系密切袁是原胚形成的重要标记遥 关键词院棉花曰体细胞胚胎发生曰细胞外基质曰扫描电镜曰透射电镜 中图分类号院S562.035.3 文献标志码院A 文章编号院1002-7807(2012)02-0167-09 SHANG Hai-hong, YUAN You-lu, LIU Chuan-liang, ZHANG Chao-jun, WU Zhi-xia, ZHANG Xue-yan, LI * Fu-guang ( , 455000, ) Research on somatic embryogenesis of L. significantly contributes to the progress in cotton genet- ic engineering breeding. Dynamic variations in structure during cotton somatic embryogenesis were observed with scanning and transmission electron microscopy. A net-like structure formed on the surface of the proembryo callus as the normal callus devel- oped into an embryogenic callus, gradually degrading with the development and formation of embryos. This phenomenon was not observed in the callus and non-embryogenic callus. The results suggest that the extracellular matrix is linked to the acquisi- tion of embryogenic competency of cotton embryogenic calli. cotton; somatic embryogenesis; extracellular matrix; scanning electron microscope ; transmission electron