泡桐脱毒组培苗生产和容器育苗技术研究.docVIP

泡桐脱毒组培苗生产和容器育苗技术研究 　　摘要泡桐是我国重要的速生用材树种,具有较高的生态、社会和经济效益。由类菌原体 MLO所引起的“丛枝病”严重影响了泡桐的生长发育和栽培的经济效益,该研究在高温结合茎尖培养脱除泡桐病毒的基础上,研究组培苗的增殖、生根培养,并且采用轻型基质容器育苗,试验表明:最佳的继代增殖培养基为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+IBA 0.2 mg/L;最佳生根配培养基为MS+IBA 0.2 mg/L+NAA 0.1 mg/L+ABT 0.4 mg/L;容器育苗中最优的基质配比为2/3稻壳+1/3锯屑。 　　关键词泡桐;丛枝病;脱毒苗;增殖培养;生根培养;轻型基质容器苗 　　中图分类号S792.43文献标识码A文章编号 1007-5739(2010)22-0182-02 　　 　　ResearchonProductionofMLO-freeSproutCultivationbyTissueCultureandTechnologyofVesselSproutCultivationofPaulownia 　　LI Feng-qingZENG Man-shengYAO Jia-baoZHOU Zhi-lanLUO Yan-lan 　　(Experimental Center of Subtropical Forestry,Chinese Academy of Forestry,Fenyi Jiangxi 336600) 　　AbstractPaulownia is an important fast-growing species and has high ecological,social and economic benefits. Witch′s broom caused by mycoplasma-like organism(MLO)has seriously affected the growth and cultivation economic benefits of Paulounia. In this research,by means of stem tip culture after temperature treatment,the MLO-free plantings were obtained,and in this basis,the proliferation,rooting and vessel sprout cultivation by light-media substrate were introduced.The results showed that the optimum subculture and proliferation culture medium was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+IBA 0.2 mg/L,the best rooting medium was MS+IBA 0.2 mg/L+NAA 0.1 mg/L+ABT 0.4 mg/L. The optimum substrate of vessel sprout cultivation was mixed with 2/3 rice husk and 1/3 sawdust. 　　Key wordsPaulownia;witch′s broom;MLO-free seedling;proliferation culture;rooting culture;vessel sprout cultivation by light-media substrate 　　 　　泡桐是我国重要的速生用材树种,具有较高的生态、社会和经济效益[1-2]。由植原体引起的泡桐丛枝病是泡桐生长的严重限制因子,通过温度和茎尖组织培养相结合从组培苗中除去病毒(MLO)的技术已经相当成熟[3-4]。随着林业事业的发展,传统的育苗方式在造林时需要一定时间恢复根系与土壤水分及养分的输送系统,成活率低。为改变这一现状,作为现代育苗体系的代表――容器育苗受到生产者的青睐[5]。容器育苗是提高种苗质量和适应种苗改变供应方式的主要手段,在我国采用此法育苗的时间虽短,但是发展非常迅速[6-7]。与传统的育苗相比,容器育苗具有很多优点,尤其该研究中采用的无纺布容器,其中填充轻型基质,不仅使农林废弃物得到合理应用,还可使苗木根系在起苗、运输、定植时避免受到损伤(折断、失水和扭曲等),具有造林成活率高,造林季节长、无缓苗期等优点[8-9]。该文就组培苗的扩繁、生根培养和容器育苗中的基质配比进行研究,以期为大规模工厂化容器育苗提供依据。 　　1材料与方法 　　1