高盐度环境下铜绿假单胞菌生产鼠李糖脂-中国科技论文在线.PDF

第4卷 第6期 418 中国科技论文在线 Sciencepaper Online 2009 年 6 月 高盐度环境下铜绿假单胞菌生产鼠李糖脂 龙旭伟，姚 斌，沙如意，章宏梓，孟 琴 （浙江大学化学工程与生物工程学系，杭州 310027 ） 摘 要：利用微生物降解海上泄漏原油成为研究热点。能分泌生物表面活性剂的细菌可以通过增加原油的生物 可利用度促进降解，但其在高盐度海水中难以存活和生长。考察了盐度、Mg2+ 、Ca2+和驯化对鼠李糖脂产生 Pseudomonas aeruginosa ZJU 细胞生长和产糖脂能力的影响。结果表明：利用与深海盐度相近的S35 合成海水 作为驯化培养基，发现驯化后的铜绿假单胞菌能较好适应高盐度环境。同时，利用菜油为碳源，在S25、S35、 S40 合成海水培养基中，铜绿假单胞菌在第 7 天的鼠李糖脂产量分别达到了 14.6 g/L、11.8 g/L和 10.8 g/L。 关键词：生物化学工程；鼠李糖脂；盐度；驯化；合成海水 中图分类号：TQ9: Q81 文献标识码：A 文章编号：1673－7180(2009)06 －0418 －5 Rhamnolipid production by Pseudomonas aeruginosa ZJU under high salinity condition Long Xuwei ，Yao Bin ，Sha Ruyi ，Zhang Hongzi ，Meng Qin (Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China) Abstract: The biodegradation of crude oil is becoming a severe problem. Biosurfacnt producer could facilitate biodegradation of crude oil via enhancing its bioavailability. This paper investigates the effect of salinity, concentration of 2+ 2+ Mg 、Ca and acclimation on the biomass and rhamnolipid productivity of Pseudomonas aeruginosa ZJU. It was found that: the acclimation of Pseudomonas aeruginosa ZJU adapted well to the high salinity condition of the S35 synthetic seawater medium which has the similar salinity as deep sea; moreover, the concentration of rhamnolipid in the S25, S35, S40 synthetic seawater medium at the seventh day reached 14.6 g/L, 11.8 g/L, 10.8 g/L respectively by using the vegetable oil as the carbon source. Key words: biochemical engineering ；rhamnolipid ；salinity ；acclimation ；synthetic seawater 和疏水性集油等物理化学方法是处理原油泄漏的常规 0 引