二氢杨梅素分离纯化和其细胞内抗氧化和体外结合胆酸盐能.PDFVIP

现代食品科技 Modern Food Science and Technology 2015, Vol.31, No.5 二氢杨梅素的分离纯化及其细胞内抗氧化和 体外结合胆酸盐能力的研究 马玲，杨继国，廖文镇，尹雪茹，邱晓斌，谭凯元，宁正祥 （华南理工大学轻工与食品学院，广州510640) 摘要：本研究通过水提重结晶法从藤茶中分离纯化得到二氢杨梅素样品，利用电子扫描电镜观察其结晶形态，并采用细胞内抗 氧化能力评价法 （cellular antioxidant activity assay，CAA ）对二氢杨梅素的细胞内抗氧化能力（CAA ）进行研究，同时在体外模拟人 体胃肠消化环境，测定了二氢杨梅素体外结合胆酸盐的能力。结果表明：利用多次重结晶法对二氢杨梅素进行分离纯化，可得到高纯 度的二氢杨梅素样品，得率为43%。细胞内抗氧化试验中二氢杨梅素的EC50 值为6.17±0.08 μmol/L，CAA 值为74.90±0.90 µmol QE/100 μmol ，表明其具有很强的细胞内抗氧化能力。二氢杨梅素对胆酸钠 （SC ），甘氨胆酸钠 （SGC ），牛磺胆酸钠 （STC ）都有较强的结 合作用，结合量分别为1.24±0.02 、0.97±0.06、1.06±0.002 μmol/mL ，由结果可知，二氢杨霉素对游离胆酸钠的结合能力最强。 关键词：二氢杨梅素；重结晶；细胞内抗氧化；体外结合胆酸盐 文章篇号：1673-9078(2015)5-157-162 DOI: 10.13982/j.mfst.1673-9078.2015.5.025 Separation, Purification, Cellular Antioxidant Activity, and In Vitro Bile Salt-Binding Ability of Dihydromyricetin MA Ling, YANG Ji-guo, LIAO Wen-zhen, YIN Xue-ru, QIU Xiao-bin, TAN Kai-yuan, NING Zheng-xiang (College of Light Industry and Food sciences, South China University of Technology, Guangzhou 510640, China) Abstract: Dihydromyricetin (DMY) was separated and purified from Ampelopsis using water extraction and recrystallization. The crystal morphology was analyzed by scanning electron microscopy and the cellular antioxidant activity (CAA) was determined by CAA assay. Meanwhile, in vitro bile salt-binding ability of DMY was determined by in vitro simulation of the human gastrointestinal environment. The results showed that separation and purification techniques with multiple recrystallizations produced high-purity DMY, and the yield was 43%. An EC50 of 6.17 ±0.08 μmol/L and CAA of 74.90 ±0.90 µmol QE/100 μmol were obtained, which indicated that DMY exhibited strong CAA. DMY also exhibi