华重楼根际土芽胞杆菌多样性的研究.docVIP

华重楼根际土芽胞杆菌多样性的研究 　　摘 要 为了解华重楼根际芽胞杆菌种群多样性信息，本研究采用稀释平板法分离福建省光泽县华重楼品种根际的芽胞杆菌，并对其进行16S rRNA序列分析。结果表明：从华重楼根际共分离到17个形态差异的芽胞杆菌菌株；16S rRNA序列分析表明，17个菌株鉴定为8个种，归属于4个属，芽胞杆菌属（Bacillus）、赖氨酸芽胞杆菌属（Lysinibacillus）、类芽孢杆菌属（Paenibacillus）和绿芽胞杆菌属（Viridibacillus），其中芽胞杆菌属的种类和数量最多。经抑菌试验分析，芽孢杆菌属的特基拉芽孢杆菌FJAT-43012对尖孢镰刀菌具有抑制作用。本研究结果表明，华重楼根际芽胞杆菌种群多样性较为丰富。本研究对芽胞杆菌资源的开发和利用提供良好的实验材料和理论基础。 　　关键词 芽胞杆菌 ；华重楼 ；根际土壤 ；多样性 　　分类号 Q938.1 　　Diversity of Culturable Bacillus Species from Paris Linnaeus 　　Rhizosphere Soil 　　CHEN Qianqian LIU Bo LIU Guohong CHE Jianmei GONG Haiyang 　　（1 Agrobiological Resource Research Institute， 　　Fujian Academy of Agriculture Sciences， Fuzhou， Fujian 350003 　　2 Ministry of Education Key Laboratory of Biopesticide and Chemical Biology， 　　Fujian Agriculture and Forestry University， Fuzhou， Fujian 350002， China） 　　Abstract The aim of this study was to determine the diversity of the cultivable Bacillus species population in the rhizosphere of Paris Linnaeus. Bacillus species were isolated from the soil samples from Guangze， Fujian Province by using dilution plating technique on Nutrient-Agar medium. 17 isolates were obtained through morphological difference， andidentified by 16S rRNA. Phylogenetic analysis based on 16S rRNA gene sequencing showed that the 17 isolates were grouped into 4 genera （Bacillus， Lysinibacillus， Paenibacillus and Viridibacillus） with 9 distinct species. All of these species were owned to the genus Bacillus. B.tequilensis FJAT-43012 possessed significantly inhibitory activities against Fusarium oxysporum. In conclusion， the result of this study showed that the population diversity of the cultivable bacteria was abundant andvarious. The findings providean important scientific base for the development and utilization of Bacillus resources. 　　Keywords Bacillus ； Paris Linnaeus ； rhizosphere soil ；diversity 　　华重楼（Paris Linnaeus）属百合科多年生草本植物，又名灯台七、七叶一枝花等，具有清热解毒、消肿止痛、凉肝定惊等功效，此外还有止血、抗肿瘤、免疫调节及保护心血管等多方面的生理活性，是一些重要中成药和新药如云南白药、宫血宁等主要成分之一[1-2]，开发和利用这一自然资源