超声空化联合无水乙醇消融兔VX2肿瘤的实验研究-影像医学与核医学专业论文.docxVIP

第三军医大学硕士学位论文 第三军医大学硕士学位论文 万方数据 万方数据 Ablation of rabbit VX2 tumor by combining microbubble-enhanced ultrasound cavitation and ethanol ablation Abstract Background: Tumor angiogenesis is believed by many study to be of great importance for tumor growth and metastasis. Fast developed neovascularization transfers requisite oxygen and nutrition to tumor cells to maintain cell activities. Disruption of tumor neovasculature may effectively inhibit or even decimate tumor cells by cutting off the supply of nutrient. Anti-angiogenesisi has been studied for decades and by now several drugs focusing on specific targets of endothelial cells have been promoted in clinical. However, the limited effects and patients dependency restricted their application. Tumor neovasculature, which is much more fragile and incomplete than that of normal tissues, is more likely to be damaged by physical effects caused by microbubble-enhanced ultrasound (MEUS). Previous studies proved that ultrasound sonication with microbubble will cause serious pathological damages (hemorrhage, thrombosis, edema et al.) to tumor neovasculature areas and would block tumor blood perfusion for as long as 24 hours. Repeated reinforcement of this effect could inhibit tumor growth and reduce metastasis, which indicates that MEUS do have the effects of anti-angiogenesis. Percutaneous ethanol ablation (PEA) is a safe, effective and minimally invasive treatment for tumor in clinical. It is the most widely used chemical ablation therapy due to the simple operating, less cost, less complication, and less damages. However, the quick washout of ethanol by tumor circulation limits the application of this therapy to small tumor which is less than 3 cm in diameter. Assumption came up that combining PEA with MEUS may increase ablation volume of ethanol by blocking blood perfusion and hence preventing washout of ethanol. So rabbits tumor models were designed to accept the combined therapy 2 with MEUS and PEA to obtain indicators