藏药蔓菁多糖的制备、组分分析及抗急性低压缺氧损伤作用的研究-药物化学专业论文.docxVIP

成都中医药大学 成都中医药大学 2013 届硕士学位论文 III III Abstract Turnip is the radix and seed of Brassica rapa L.. The drug is collected in autumn when the radix and seed are ripe. Turnip is also called NIUMA (from Tibetan medicine’s classics Four-Volume Medical Code) in Tibetan herb, and is generally called MANJING, YUANGEN in Tibet. In traditional Tibetan Medicine theory, turnip has the effect of detoxification and nourishing. It is suitable for treatment of all kinds of poisons, ‘Long’ illness and body weakness. At present, the Tturnip has not established a complete quality control method, turnip polysaccharides have also not been reported effects on mice acute hypoxia. Purpose To optimize extraction and purification procedures of antioxidant activity polysaccharides from Brassica rapa; Analysis of turnip in the monosaccharide composition of polysaccharide; To study the protective effects of turnip polysaccharide on normobaric hypoxia and acute hypoxic hypoxia injury; To establish a method for quality control of the turnip in order to provide the evidence for health food developing on turnip. Methods With the crude polysaccharides yield,the content and antioxidant activity of total polysaccharides as indicators,orthogonal design was used to optimize extraction technology by taking extraction times,solid-liquid ratio and extraction time as factors;With the removal rate of pigment and the loss rate of polysaccharides as comprehensive evaluation index,effects of bleaching temperature,adsorption time and activated carbon dosage on decoloration technology were investigated by orthogonal test. Using the high performance liquid chromatography to analyze the monosaccharide composition of polysaccharide. Using normobaric hypoxia test and acute hypobaric oxygen chamber hypoxia experiment to study the protective effect of turnip polysaccharide on anoxic injury in IV IV mice. Using the phenol-sulfuric acid method to determine the total polysaccharide content of turnip. Results