多电极电生理系统建立、完善及应用-生物工程专业论文.docxVIP

本论文所做的工作来源于国家自然科学基金课题（No：视 网膜神经节细胞电活动的时空编码研究，为该项研究建立了一个稳定的多 电极电生理实验系统。该项研究课题有助于通过对视觉信息处理机制的认 识，从而阐明动态神经元群体活动在视觉信息加工传递中的作用；同时有 助于通过对生物神经元网络中信息传递过程的认识，对人工视觉系统功能 的开发做出贡献。 关键词：视网膜，神经节细胞，多电极记录 CONSTRUCTION AND APPLICATION OF THE MULTI-CHANNEL ELECTROPHYSIOGICAL RECORDING SYSTEM Abstract The first stage of visual information processing occurs in the retina. Visual stimuli excite the photoreceptors, which convert the visual stimuli into electrical signals. These signals are then propagated through the retinal circuitry to the output neurons (the ganglion cells) and are relayed to the central visual system in the form of the action potentials via optic nerve fibers. Multi-electrode recording technique is an important way to study the retinal information processing. With the application of this technique, we can record the activities of a group of neurons simultaneously and analyze the spatio-temporal patterns of the neuronal group. Through synchronized recording of a group of ganglion cells’ firing activities in response to specific light stimulation, we can know better on how the ganglion cells encode and transmit visual signals in a concerted way. Before we use this technique, the fist thing is to set up a multi-electrode recording system, which should fit the special requests of biological experiments. In the present study, the system was established through two steps. First, we set up a multi-electrode recording system, which owns four abilities: display light stimuli to the retina, perfuse the retina with physiological solution, keep the temperature of the perfusate and the tissue stable, record the activities of a group of neurons simultaneously and store them in the computer. Second, we analyzed the results of the experiments performed on the multi-electrode recording system, to testify if this system can record the activities of a group of neurons precisely. This work was supported by grants from the National Natural Science Foundation of China (NSFC, No.