多肽金属纳米簇的制备及其用于生物分析检测研究-化学专业论文.docx

PAGE PAGE IV 摘要 不仅可以消除光源或检测器的漂移以及环境条件等其他因素对检测结果的影响 和干扰，还实现了紫外灯下的可视化检测。对今后磷酸化疾病的发现与早期诊 断和对磷酸化相关疾病药物及抗癌药物的筛选与研发具有潜在应用。 5、以同一激发不同发射的两种生物矿化的金属纳米簇为双发射同时检测荧 光探针，实现对蛋白激酶 PKA 和蛋白激酶 CK2 的同时检测。以蛋白激酶 PKA 和蛋白激酶 CK2 的底物多肽为模板分别制备发蓝光的 peptide-Cu NCs 和发红光 的 peptide-Au NCs。当加入一种蛋白激酶时，只有相应的多肽底物制备的金属纳 米簇的荧光发生恢复，另一种金属纳米簇荧光强度保持不变，则可对蛋白激酶 进行单独检测，并消除环境等因素对检测结果的影响，达到比率荧光检测的效 果，对蛋白激酶的鉴别与筛选具有重要意义。当同时加入两种蛋白激酶时，两 种金属纳米簇荧光同时增强，从而达到对两种蛋白激酶的同时检测。这种以两 种金属纳米簇为荧光探针的方法，填补了金属纳米簇在同时检测领域应用研究 的空白，以其操作简单、灵敏度高且可视化的特点，可以达到对两种蛋白激酶 的分别检测及同时检测，从而对研究与具体蛋白激酶有关的疾病及癌症有潜在 应用。 关键词：金属纳米簇；磷酸化；蛋白激酶；抑制剂；羧肽酶 Y；比率荧光；同时 检测 Abst Abstract ABSTRACT Metal nanoclusters as a new class of metal nanoclusters have attracted great attention in recent years for their unique and intriguing physical and chemical properties. However, strong reducing agents which would disrupt the biological function of models were required in these synthesis processes and further complex chemical steps are needed to purify and functionalize the clusters for their later biologic applications. Therefore. it’s necessary to find out a simple method to synthesize metal nanoclusters without any reducing agents. Protein kinase which catalyzes protein phosphorylation and has a crucial role in treatment of diseases including cancer. Therefore, the establishment of high sensitivity and simple operation detection method of protein kinase not only provide the basis for the early diagnosis of cancers, but also helps to treat cancer-related phosphorylation of discovery and screening of new drugs. In this thesis, we synthesized metal nanoclusters by a one-step peptide method without any strong reducing agents, and designed a series of routes to detect the protein kinase which would help us find out a simple and sensitive method. The main work of my graduation thesis will be written in details in the sections below. A label-free, sensitive and simple method to detect protein kinase based on the selective aggregation of phosphorylated peptide-gold nanoclusters (pepti