桂枝配方颗粒饮片水煎剂HPLC指纹图谱相关性的研究.docVIP

桂枝配方颗粒饮片水煎剂HPLC指纹图谱相关性的研究 　　摘要：目的 采用HPLC对桂枝配方颗粒、饮片和水煎剂的指纹图谱进行相关性研究，考察三者之间化学成分的差异。方法 采用Diamonsil C18色谱柱（4.6 mm×250 mm，5 ?m），流动相为乙腈-0.1%磷酸，梯度洗脱，检测波长280 nm，流速1.0 mL/min，柱温30 ℃。分别建立10批桂枝配方颗粒、10批饮片、10批水煎剂的指纹图谱。结果 桂枝配方颗粒HPLC指纹图谱中的12个共有峰均可在水煎剂中得到追踪，有10个可在饮片中得到追踪，并指认出原儿茶酸、香豆素、肉桂酸3种成分。结论 桂枝配方颗粒与水煎剂的主要化学成分组成基本相同，其共有成分含量比例接近。 　　关键词：桂枝配方颗粒；饮片；水煎剂；高效液相色谱法；指纹图谱；相关性；肉桂酸 　　DOI：10.3969/j.issn.1005-5304.2017.06.022 　　中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2017）06-0087-04 　　Abstract： Objective To study the correlation of fingerprint chromatograms of Cinnamomi Ramulus formula granules， decoction pieces and water decoction by HPLC； To investigate the difference of main chemical constituents among different forms. Methods The Diamonsil C18 column （4.6 mm × 250 mm， 5 ?m） was used with mobile phase of acetonitrile and 0.1% phosphoric acid at the flow rate of 1.0 mL/min， with detection wave of 280 nm and temperature of 30 ℃. The detection of 10 batches of Cinnamomi Ramulus formula granules， 10 batches of decoction pieces and 10 batches of water decoction were established respectively. Results Totally 12 peaks in the HPLC fingerprint chromatogram from 10 batches of formula granules could be tracked in the water decoction； 10 peaks in the HPLC fingerprint chromatogram could be tracked in the decoction pieces. Three components， such as protocatechuic acid， coumarin and cinnamic acid were verified. Conclusion The main chemical components of Cinnamomi Ramulus formula granules and water decoction are basically the same， and the common component contents have similar proportion. 　　Key words： Cinnamomi Ramulus formula granules； decoction pieces； water decoction； HPLC； fingerprint chromatogram； correlation； cinnamic acid 　　桂枝?檎量浦参锶夤?Cinnamomum cassia Presl的干燥嫩枝，具有发汗解肌、温通经脉、助阳化气的功效，用于治疗风寒感冒、脘腹冷痛、血寒经闭、关节痹痛、痰饮、水肿、心悸、奔豚[1]。桂枝主要含有挥发油类、有机酸类、多糖类、甾体类、香豆素类及鞣质类等成分，具有抗菌、抗炎、抗病毒、解热、镇 　　静、镇痛、抗惊厥、抗肿瘤、利尿等药理作用[2-4]。肉桂酸和原儿茶酸属于有机酸类成分，肉桂酸具有抗菌、升高白细胞、利胆、抗突变、诱导人肺癌细胞恶性表型逆转和抗侵袭等药理作用，原儿茶酸是抗炎、抗菌的活性成分[5]。 　　桂枝配方颗粒是以符合炮制