全反式维甲酸对腹膜透析相关性腹膜纤维化大鼠转化生长因子β1、结缔组织生长因子、α..docVIP

全反式维甲酸对腹膜透析相关性腹膜纤维化大鼠转化生长因子01、结缔组织生长因子、a平滑肌肌动蛋白表达的影响 ［ ］目的探讨全反式维甲酸（ATRA）在腹 膜透析中的抗腹膜纤维化作用。方法选择40只雄性 SD大鼠，编号后随机分为对照组（腹腔注射生理盐水 10 mL/d+与药物等体积的磷酸盐缓冲液稀释的二甲亚 砜）、模型组（腹腔注射4.25%的腹透液10 mL/d+与药 物等体积的磷酸盐缓冲液稀释的二甲亚砜）、实验1 组（腹腔注射4.25%的腹透液10 mL/d+ATRA 2 mg/kg）、 实验2组（腹腔注射4.25%的腹透液10 mL/d+ATRA 5 mg/kg）,每组各10只；四组大鼠正常喂养4周后检 测腹膜功能，并采用免疫组化法检测大鼠腹膜纤维化 相关细胞因子的变化情况。结果实验第4周，模型 组和实验1组的腹膜功能指标超滤量、透析液尿素浓 度/血浆尿素浓度、初始腹透液葡萄糖浓度/透出液葡 萄糖浓度测定值较对照组均有不同程度的下降（P 0.05）。与对照组比较，模型组、实验1组、实验2组 的脏层腹膜组织中转化生长因子邛1 （TGFH）、结 缔组织生长因子（CTGF）、a平滑肌肌动蛋白（ct -SMA） 免疫组化检测结果均发生显著的变化（P ［关键词］全反式维甲酸；腹膜透析；抗腹膜纤维 化；细胞因子 ［ ］R692.5 ［ ］A ［ ］ 1673-7210 (2015) 08 (a) -0004-04 Influence of all trans retinoic on expression of TGF- 卩 1， CTGF， a -SMA in peritoneal dialysis related peritoneal fibrosis rat LI Minzhi XIONG Yanyi SHI Weiwen ZHANG Guarding ZHUO Huaqin Department of Nephrology， the Third People’s Hospital of Dongguan City， Guangdong Province, Dongguan 523320， China ［Abstract］ Objective To investigate the effects of all trans retinoic acid (ATRA) in anti peritoneal fibrosis of peritoneal dialysis. Methods Forty healthy male SD rats were selected, after numbered they were randomly divided into control group (intraperitoneal injection 10 mL/d normal saline + phosphate buffer diluting dimethyl sulfoxide with same volume as medicines)， model group (intraperitoneal injection 4.25% peritoneal dialysis fluid 10 mL/d + phosphate buffer diluting dimethyl sulfoxide with same volume as medicines)， experiment group 1 (intraperitoneal injection 4.25% peritoneal dialysis fluid + ATRA 2 mg/kg)， experiment group 2 (intraperitoneal injection 4.25% peritoneal dialysis fluid 10 mL/d + ATRA 5 mg/kg)，with 10 rats in each group； peritoneal membrane function of rats in four groups after 4 week were detected, changes of peritoneal fibrosis related cytokines in rats was detected by immunohistochemical method. Results In the fourth week, compared with the control group， peritoneal function indexes of UF， D/Purea， D1/D0 in model group and experiment group 1