基于肝微粒体和肝S9成分的食品接触材料中五氯苯酚体外代谢分析.PDF

7 1 Vol. 7 No. 1 2016 1 Journal of Food Safety and Quality Jan. , 2016 S9 龚 强, 丁 利, 朱绍华, 成 婧, 焦艳娜, 付善良, 王利兵* ( , , , 410004) 摘 要: 目的 , (pentachlorophenol, PCP)方法 S9 2 , , PCP(Cl4BQ)2 -(GC-MS), PCP 结果 0.75 mg/mL4 h 结论 2 PCP , PCP Cl4BQ, S9 关键词: ; ; ; S9 ; -; Metabolic study of pentachlorophenol in food contact materials by liver microsome and liver S9 fraction * GONG Qiang, DING Li, ZHU Shao-Hua, CHENG Jing, JIAO Yan-Na, FU Shan-Liang, WANG Li-Bing (Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau, State Key Laboratory of Food Safety Testing, Hunan Key Laboratory of Food Safety Science Technology , Changsha 410004, China) ABSTRACT: Objective To analyze the metabolite of pentachlorophenol (PCP) through simulating metabolism in vivo. Methods Liver microsomes and liver S9 fraction were used as two kinds of in vitro metabolism reagents to simulate in vivo metabolic pattern. The method for the determination of PCP and tetrachloro-1,4-benzoquinone (Cl4BQ) was established by gas chromatography-mass spectrometry (GC-MS) through optimizing the metabolic reaction conditions, and th