白藜芦醇恢复去分化正常髓核细胞表型的试验研究-OpenRepository.PDF

2013 5 27 5 547 resveratrolRES 6 1357 β- senescence-associated β-galactosidaseSA-β-gal 1 1 7 A RES B 2 1silent mating type information regulation 2 homolog 1SIRT1- RNA small interfering RNAsiRNA+ RES C -siRNA+RES D E Western blot SIRT1Aggrecan PCR SIRT1 mRNA SA- β-gal 48 h P 0.05SIRT1 Aggrecan E A P 0.05A B 3 P 0.05 C SIRT1 mRNA BD P 0.05Aggrecan B D P 0.05 RES SIRT1 RESTORING PHENOTYPE OF DEDIFFERENTIATED NORMAL NUCLEUS PULPOSUS CELLS BY RESVERATROL/SHEN Jieliang, HU Zhenming, ZHONG Xiaoming, WANG Dawu, XU Linfei. Department of Orthopaedics, First Ailiated Hospital of Chongqing Medical University, Chongqing, 400016, P.R.China. Corresponding author: HU Zhenming, E-mail: zhenminghu62@ Abstract Objective To investigate the effects of in-vitro monolayer culture and three-dimensional (3-D) alginate microsphere culture on the differentiation of normal human nucleus pulposus cells (NPCs), and to discuss the regulatory mechanism of restoring the phenotype of dedif erentiated NPCs by culturing resveratrol (RES) in 3-D alginate microsphere. Methods Normal human nucleus pulposus tissues were harvested for culture and identii cation of NPCs from 6 patients with burst lumbar vertebra fracture. NPCs at passages 1, 3, 5, and 7 in the in-vitro monolayer culture were harvested to observe the morphology, cell aging, and proteoglycan expression. The cell proliferation rates of NPCs at passage 1 in-vitro in monolayer culture and in 3-D alginate mic