HPLC比较不同产地生药八角莲中鬼臼类成分含量.PDFVIP

HPLC 比较不同产地生药八角莲中鬼臼类成分含量 肖昌钱 1 2 3 1* ，杨淑贞 ，单文 ，周长新 (1.浙江大学药学院现代中药研究所，杭州 310058 ；2.浙江天目山国家自然保护区管理局科研 所，杭州 311311 ；3.江西省庐山植物园, 江西 九江 332900) 摘要：目的 比较不同产地的八角莲中鬼臼毒素和4’-去甲鬼臼毒素含量。方法 采用 RP-HPLC ，色谱柱C18 (250 mm ×4.6 mm ，5 μm) ，流动相为甲醇-0.1 mol·L-1 K HPO (50 ∶50) ，流速 1.0 mL·min-1 ，检测波长207 nm ，柱温25 ℃。结果 鬼臼毒 2 4 素的线性范围 20~400 ng ，r=0.999 9 ，回收率 100.4%，RSD=1.2%；4’-去甲鬼臼毒素的线性范围 2~20 ng ，r=0.999 8 ，回收 率 101.6%，RSD=2.5% 。结论 该方法准确、可靠、简便，不同地域的生药八角莲中鬼臼类成分含量差异明显。 关键词：八角莲；鬼臼毒素；4’-去甲鬼臼毒素；高效液相色谱法；含量测定 中图分类号：R284.1; R917.101 文献标志码：B 文章编号：1007-7693(2009)08-0659-03 Comparison of Podophyllotoxin and 4’-Demethylpodophyllotoxin Contents in Rhizoma et Radix Dysosmae from Different Areas by HPLC 1 2 3 1* XIAO Changqian , YANG Shuzhen , SHAN Wen , ZHOU Changxin (1.Institute of Modern Chinese Medicine, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China; 2.Institute of Scientific Research, Administration Bureau of National Natural Protection Area of Zhejiang Tianmu Mountain, Hangzhou 311311, China; 3. Jiangxi Lushan Mountain Botanical Garden, Jiujiang 332900, China) ABSTRACT: OBJECTIVE To compare the contents of podophyllotoxin and 4’-demethylpodophyllotoxin in Rhizoma et Radix Dysosmae of different areas. METHODS The assay was performed on phenomenex C18 (250 mm ×4.6 mm, 5 μm). The mobile phase was MeOH-0.1 mol ּL-1K HPO (50:50). Flow rate was 1.0 mL ּmin-1. Wavelength was 207 nm, and the temperature of column 2 4 was 25 ℃. RESULTS The calibration curve was linear in the range of 20-400 ng for podophyllotoxin (r=0.999 9). The average recovery was 100.4% (RSD=1.2%). And the liner range of demethylpodophyllotoxin was 2-20 n