烟色烟管菌诱变提高漆酶产量的-研究.pdfVIP

Abstract whichcan inthefieldsof Laccasehavebroad textile， applicationprospects apply andSOon．TheWhiterot from food，paper,medicine，agriculture fungi isthemostefficient for laccase laccase．However,the Basidiomycotina producer fromwildstrainsistoolowto to inindustrial activity adaptproduct produce．This choose tomutateUVand inorderto paper Bjerkanderafumosa by 60Co吖rays the s仃mnsof can atheoreticalbasisforthe yielding laccase，whichprovide selecting industrial production． Mutate thebestmutationtimeWas1 UV 80s，mutation Bjerkanderafumosaby rateWas33．3％．SelectedthestablestrainZW二7whichlaccase Was activity320．2U／L， 27．8％that the time highestenzymeproduction improved compared谢thoriginal．The Was Wastheninth wasearlierin2 ZW-7 by days，which days．The mutagenized with radiationandthebestradiationdoseWas rate treating60Co吖ray 500Gy,mutation Co-11whichlaccase Was3 Was41．7％．Selectedthestablestrain activity80．5U／L，that Was 18．9％oftheZW-7and51．8％ofthe strain．Theenzyme improved original highest timeWas