缺氧对HUVECS生物学特性VEGFR表达影响的体外实验-研究.pdfVIP

VEGF mRNA was not significant (P0.05) between time at 0h to 12h and 24h to 48h. Conclusion: HUVEC subjected to hypoxia exhibited reduced cell migration, proliferation and tube formation. Hypoxia stimulated VEGF mRNA expression but decreased VEGFR1 and VEGFR2 protein levels in endothelial cells. A mechanistic explanation is that VEGFR1 and VEGFR2 protein levels were depleted whereas VEGFR1:VEGFR2 ratio is substantially increased during hypoxia to block VEGF stimulated and VEGFR2 regulated endothelial responses to maximize cell vi