紫外分光光度法测定保健食品中总蒽醌的含量-食品安全质量检测学报.PDFVIP

7 4 Vol. 7 No. 4 2016 4 Journal of Food Safety and Quality Apr. , 2016 1 1 1 2* 潘小红 , 钟庆元 , 刘玉玲 , 周利平 (1. , 410001; 2. , 410001) 摘 要: 目的 方法 , 结果 , 30 min, , , 518 nm 97.08%, 3.96%(n=9), 1, 8-0.0020~0.0252 mg/mL (r2=0.9996)40~75 min 结论 , 关键词: ; ; ; 1, 8- Determination of total anthraquinones in health foods by ultraviolet pectrophotometry 1 1 1 2* PAN Xiao-Hong , ZHONG Qing-Yuan , LIU Yu-Ling , ZHOU Li-Ping (1. Hunan Institute for Food and Drug Control , Changsha 410001, China; 2. Hunan Science and Technology Exchange Center, Changsha 410001, China) ABSTRACT: Objective To establish a method for determination of total anthraquinone in health foods by ultraviolet pectrophotometry. Methods The methods for determination of total anthraquinones were optimized by studying on the instrument conditions, color stability, extraction methods, extraction solvents and hydrolysis acid choice and so on. Results Samples were extracted with mixed acid as extraction agent, with the direct heat reflux for 30 min, and then extracted by ether. Using mixed alkali to present color reaction, samples were detected at the wavelength of 518 nm. The average recovery of the detection was 97.08%, and the relative standard deviation (RSD) was 3.96%. 1, 8-dihydroxy anthraquinone had a