燕麦转录因子BTF3的电子克隆和生物信息学分析ElectronicCloning.PDFVIP

网络出版时间：2016-05-29 17:04:38 网络出版地址：/kcms/detail/46.1068.S1704.004.html 研究论文 ResearchArticle 燕麦转录因子BTF3 的电子克隆和生物信息学分析 * 邵沾沾 王永生 福建农林大学, 作物科学学院, 福州,350002 *通讯作者,shaozhanzhan8905@163.com 摘 要 运用电子克隆的方法获得一个燕麦BTF3基因的完整的cDNA 序列。利用生物信息学方法对其编 码的蛋白的一，二，三级结构和功能进行预测和分析。结果表明：该基因cDNA 全长为870bp，开放阅读 框为531bp，编码176个氨基酸，是一种亲水的不稳定的蛋白，定位在核内，拥有一个NAC保守结构域， 与小麦等其它植物高度相似。为燕麦BTF3基因的克隆，功能分析和应用提供了参考。 关键词 燕麦,BTF3, 电子克隆, 生物信息学 Electronic Cloning and Chatacterization of BTF3 Gene from Avena SativaUsingBioinformaticsTools * ShaoZhanzhan WangYongsheng College of Crop Science,FujianAgriculture andForestry University,Fuzhou,350002 *Corresponding author,shaozhanzhan8905@163.com Abstract It had been cloned completely that cDNA of Avena sativa BTF3 gene by electronic cloning. The function and several charactersofthe amino acidencoded byAvenasativaBTF3 gene such asprimary,secondary and tertiary structure were predicted and analyzed by bioinfomatics analysis. The results showed that the full-length of cDNAwas 870 bp, including a complete ORF encoding 176 amino acid which washydrophilicity and instability. It also was predicted that the protein were located in nucleus and contained an typical NAC domain, which was highly conservative comparing with BTF3 from other plants such as Triticum aestivum.The aboveresultswillprovide some referencesfor experimentalcloning,identification andapplication. Keywords AvenasativaL,BTF3,Electronic cloning,Bioinformatics BTF3 即基本转录因子 3 (Basic transcription factor 3, BTF3)，为转录因子NAC (nascen-t polypept ide-associated complex)中两个亚基α、β的β亚基，最初是在人类研究中被发现的，它通过与RNA 聚合酶II 结合，参与调控转录过程(Zheng et al., 1987)，它是转录起始所必需的基本转录因子。但是随后的体外实验 研究证明BTF3对于转录起始并非是必需的，PEA3 家族成员可以激活或者提高食管癌的发病概率，食管 癌晚期ERK 的信号传递就会变强(El-Tanani et