食用合成色素亮蓝荧光光谱特性研究-毕业论文（设计）.docxVIP

食用合成色素亮蓝荧光光谱特性研究 摘 要: 应用荧光光谱法研究了浓度、pH、氧化剂、还原剂、食用酸、金属离子、猝灭剂、乙醇介质和其他几种食用合成色素对亮蓝色素荧光光谱的影响。结果表明：亮蓝色素在mol/l 、pH=9.87、激发波长340nm、发射波长450nm时荧光强度最强。高锰酸钾和重铬酸钾使亮蓝的荧光强度下降，而氯酸钾基本不变。还原剂使亮蓝的荧光强度下降。、、、、、苹果酸、柠檬酸、山梨酸钾、苯甲酸钠、HYPERLINK /view/861232.htm \t _blank 苋菜红、日落黄、在误差允许范围内使亮蓝的荧光强度略有下降，苹果酸对亮蓝的影响较大。亮蓝可以与氯酸钾、、、、、、柠檬酸、山梨酸钾、HYPERLINK /view/861232.htm \t _blank 苋菜红、日落黄、胭脂红共存。而高锰酸钾、重铬酸钾、亚硝酸钠、亚硫酸钠、盐酸羟胺则只能加微量。乙醇存在时，随着乙醇浓度的增加，亮蓝荧光强度下降。在最佳条件下得出荧光光谱法测定亮蓝的标准曲线方程为=1.3+28.89,最佳线性范围为至2mol/l相关系数为γ=0.98 ,检出限为D=7.4mol/l,对样品醒目饮料亮蓝测定的相对标准偏差为7.6%，加标的回收率为95.0%～108.7%。 关键词：亮蓝；荧光光谱法；合成色素 Study on thebrilliant blue fluorescence spectra of synthetic edible pigment ABSTRACT：Application of fluorescence spectroscopy study of the concentration, pH, oxidant, reductant, edible acid, metal ions, quenching, the ethanol medium and several other edible synthetic pigment effect on blue pigment fluorescence spectra. The experimental results show that: the blue pigment in mol/l, pH=9.87 the excitation wavelength of 340nm, emissionwavelength of 450nmthe strongest fluorescence intensity. Potassium permanganate and dichromate the fluorescence intensity of Coomassie brilliant blue drop, and potassium chlorate is basically unchanged. The reducing agent the fluorescence intensity of Coomassie brilliant blue drop.，，，，, malic acid, citric acid, tartaric acid, potassium sorbate, sodium benzoate, tartrazine, sunset yellow, in the range of allowable error the fluorescence intensity of Coomassie brilliant blue slightly, effects of malic acid on the big blue. Brilliant blue with potassium chlorate, ，，，，, citric acid, potassium sorbate, amaranth, carmine, sunset yellow coexistence. And potassium permanganate, potassium dichromate, sodium nitrite, sodium sulfite, hydroxylamine hydrochloride can only add trace. The presence of ethanol, as the concentration of ethanol increased, decreased the bright blue fluorescence intensity. The standard curve equation is obtained by Coomassie brillian blue fluorescence spectroscopy in the