兔骨髓间充质干细胞同种异体修复关节软骨缺损的实验--研究.pdf

c…自大学硕士学忆沧立 ABSTRACT and stem Objective：Isolatingculturinghigh·grademesenchymal cellsfromRabbitsbonema丌owandthendifferentiateintochondroblast To effectof invitro evaluatethe bonemarrow stroroal mesenchymd Sodium full-thickness cells(BM—MSCs)withhyaluronatehealing defectsofrabbitknee cartilage Methods：1．Bonemarrowofrabbitswasobtainedfrom proximal tibiasterilesternum needleand by Reformingensity puncture gradient was cultivationutilizedtocultureBM·MSCsitculturedin centrifugation wi廿l theDMEMlow 15％fetalbovineserum． containing glucose thecellsurfacemarkersflow in 3 2． Analyze by cytometrypassage intotwo BM-MSCsfromthesecond weredivided passage groups：the induction the control medium；the groupwere，addingchondrogenic were thecommonmediumThe oftheculturedcells groupadding growth observedAlcianblue wereusedtodetectthesecretionand was staining of inextracellularmatrix．3 glycosaminoglycan Eighteen expression rabbitsweredividedintothree defectsofthe groupsrandomly．the