番茄延伸体复合物核心亚基SlELP2的克隆及功能研究-生物学专业论文.docx

重庆大学硕士学位 重庆大学硕士学位论文 英文摘要 PAGE PAGE VI ABSTRACT The multi-subunit complex Elongator is a histone acetyltransferase and highly conserved in eukaryotes. Elongator is composed of two parts: ELP1-3 forms the core subcomplex, while ELP4-6 forms the accessory subcomplex. SlELP2 is one of core subunits of Elongator obtained in tomato. Elongator has functions in RNAPII-mediated transcription and translation through the acetylation of histone H3 and H4 in chromatin, the modification of certain tRNAs and the acetylation of a-tubulin. In plant, functional study of Elongator only carried out in Arabidopsis thaliana and has not been reported in other species. In Arabidopsis thaliana, mutation of any subunit of Elongator resulted in pleiotropic phenotypes, such as narrow leaves, inhibited root growth, reduced fertility and ABA hypersensitivity. In addition, Elongator has a role in regulation of various hormones such as JA, ET, and SA, implying it is involved in the regulation interactions of hormones and be essential for maintaining hormonal balance and appears to be essential in defense of biotic and abiotic stress responses. Here, we study the function of Elongator in fleshy fruit plant through RNAi-mediated down-regulation of ELP2 gene expression in tomato，which is aiming to further figuring out the role of Elongator in plant growth, fruit mature and development. The main points and results of our study are described as following, Clone full length of SlELP2 cDNA and expression profile analysis of genes According to the ELP2 homologous gene sequence of Micro-Tom in GenBank, we designed the primers of full length of SlELP2 coding sequence and cloned a 2382bp cDNA, which has one base difference to previous sequence of Micro-Tom. It consists of a 2190bp ORF and encodes a 729 amino acid residues, with a molecular mass of 80.4 kDa and isoelectric point of 6.02. We renamed this gene as SlELP2. Quantitative real-time PCR analysis indicated that SlELP2 was presented co