大豆脂肪酸组分遗传及QTL研究-作物遗传育种专业论文.docx

A genetic map based on SSR markers is constructed with F2 population from the cross Jidou 12× Heidou. It spreads over 14 linkage groups and contains 66 SSR markers. 14 quantitative trait loci (QTLs) for fatty acid content were found in this population. One QTL accounted for 52% of variation in palmitic acid content. Three QTLs accounted for 7%-25%， explained 32% of total phenotypic variation for Stearic in F2, 31% in F2:4. Four QTLs accounted for 4%-20%，explained 31% of total phenotypic variation for Oleic in F2, 24% in F2:4. Two QTLs accounted for 12%-24% for Linoleic content. explained 36% of total phenotypic variation for Oleic in F2, 11% in F2:4. Four QTLs accounted for 9%-20% for Linolenic. 4 QTLs were confirmed in more than two environment,which controlled the Palmitic on D1a（r2=52%）,and Stearic on C2 (r2=7%),and Oleic on A2 (r2=19%),and Linoleic on D1a (r2=24%) . QTL of soybean fatty acid content in four different environments was analyzed by mixed linear model approach. 4 QTLs with additive effect, dominance effect effects for fatty acid content were detected and these effects were all effected by environment interaction. They control Stearic, Oleic, Linoleic and Linolenic. The heredity value of effected by environment were 6.05%,2.96%,5.54%, and 2.89%. Two pairs of QTL with epistemic effects for fatty acid content in this population were detected. They were detected from QTL× environment interaction. A pairs of QTL on LG-F between sat_133 and satt663 and LG-O between satt358 and satt445 control Stearic. One pair of QTL on D1a between sat_353 and sat_468 and A1 between satt684 and sat_368 control Linoleic. Key words： Soybean， Germplasm resources， Composition of fatty acid， QTL Mapping， QTL × environment interaction， Epistatic effects VI 学位论文原创性声明 本人所提交的学位论文《大豆脂肪酸组分遗传及QTL分析》，是 在导师的指导下，独立进行研究工作所取得的原创性成果。除文中已 经注明引用的内容外，本论文不包含任何其他个人或集体已经发表或 撰写过的研究成果。对本文的研究做出重要贡献的个人和集体，均已 在文中标明。 本声明的法律后果由本人承担。 论文作者（签名）： 指导教师确认( 签名) ： 年 月 日 年 月 日 学位论文版权使用授权书 本学位论文作者完