丹参酮ⅡA对大鼠脑缺血再灌注损伤磷酸化p38MAPK和MMP-9表达的影响-神经内科专业论文.docx

PAGE PAGE 10 灌注后细胞凋亡，抑制 MMP-9 表达，降低血脑屏障通透性，可能与抑制 p38MAPK 信号通路有关。 关键词：缺血再灌注；磷酸化 p38MAPK；MMP-9；凋亡；血脑屏障； 丹参酮ⅡA Effects of TanshinoⅡA on expressions of phosphorylated p38MAPK and MMP-9 and neural cell apoptosis following cerebral ischemic reperfusion injury in rats ABSTRACT Objective To study effects of Tanshinone ⅡA (TanⅡA) on neural cell apoptosis and the permeability of the blood-brain barrier following cerebral ischemia reperfusion (IR) injury rats, and explore the releationship between theirs and p38 mitogen-activated protein kinase pathway. Method 60 SD rats were randomly divided into four groups, which were sham operated group, IR group, low dose TanⅡA treated group and high dose Tan ⅡA treated group. The focal middle cerebral artery occlusion (MCAO) model was made in rats with suture-occluded method. Low dose and high dose groups were pretreated with low and high does TanⅡA, for 3d, before MCAO. After 120min MCAO following 24h reperfusion, We investigated the expressions of phosphorylated p38MAPK and MMP-9 in using immunohistochemistry technique in the frontal and parietal cortex; Neural cell apoptosis were investigated with TUNEL and HE staining; And blood-brain barrier(BBB) was investigated by the contents of evens blue (EB); The volume of cerebral infarction was observed by TTC. Results ⑴ Compared with sham operated group, phosphorylated p38MAPK and MMP-9 increased in IR group; Compared with IR group, high and low dose Tan ⅡA treated groups dose-dependently decreased expression of phosphorylated p38MAPK and MMP-9 ( P 0. 05); ⑵Compared with sham operated group, cell apoptosis and pathomorphological change of ischemic area were distinctly increased in IR group; Compared with that of IR group, low and high Tan ⅡA treated groups dose-dependently reduced neural cell apoptosis and pathomorphological change (all P0.05); ⑶Compared with sham operated group, the contents of EB increased at 24h of reperfusion . Compared with IR group, low and high