磁共振弥散加权成像技术预测鼻咽癌放射敏感性的价值及其机制分析-内科学(血液)专业论文.docx

PAGE PAGE 4 Values of magnetic resonance diffusion-weighted imaging on prediction of radiosensitivity in nasopharyngeal carcinoma and its mechanisms Abstract Background and Purpose The effect of radiotherapy for nasopharyngeal carcinoma (NPC) is closely related to radiosensitivity in the carcinoma, but currently, there is still no effective way to predict this radiosensitivity. Magnetic Resonance Diffusion-weighted imaging (MR-DWI), a molecular and functional imaging technique providing apparent diffusion coefficient (ADC) as a quantitative parameter, is somewhat correlated with responses to radiotherapy and chemotherapy for cervical cancer, liver cancer and head and neck cancer. This study intends to explore the predictive value of MR-DWI for detecting radiosensitivity in nasopharyngeal carcinoma and relevant mechanism with animal models and patients, and to provide an experimental basis for its clinical application. Materials and Methods 1.Nude mice bearing human nasopharyngeal carcinoma were randomly divided into control group and irradiation group (a single dose of irradiation with 15Gy). ADC values were measured before irradiation(ADC0) and the next day after irradiation (ADC1).The ADC value change(ΔADC) after irradiation was indicated by (ADC1-ADC0) / ADC0*100%, and the change in HYPERLINK /dict_result.aspx?searchword=%e7%a7%bb%e6%a4%8d%e7%98%a4amp;tjType=sentenceamp;styleamp;t=xenograft xenograft volume (ΔVx ) at X days after irradiation by (Vx-V0)/ V0*100%. The difference in the volume change between control group and irradiation group was defined as radiation-related volume change,δVX = ΔVxc-ΔVxt. 2. Hypoxia imaging of nasopharyngeal carcinoma xenografts with 99mTc-HL91 SPECT was conducted in nude mice to analyze the correlation of the ADC value with the radioactivity ratios of target-to-nontarget. 3. In addition, nude mice with nasopharyngeal carcinoma xenografts were randomly divided into non-irradiated group and five other groups (1d, 2d, 3