粗壮脉纹孢菌降解富含纤维的农副产品及其降解产物发酵产乙醇的研究-营养与食品卫生学专业论文.docx

Abs Abstract ABSTRACT In this paper, high vitality cellulose was produced by Neurospora crassa on low-cost agro-industrial residues in rich of fibers in solid-state fermentation. Neurospora crassa, which could produce high amount of cellulase, was isolated and mutated from the traditional fermented food in Jiangxi province. The composition of culture medium were optimized to enhance the vitality of cellulose. Effect of culture conditions on reducing sugar from fibrous materials were investigated. And the compositions of fermentable sugar, which came from the degradation product under this optimal culture medium, were analyzed by gas chromatography. This work aim to select the best yeast strain and fermentation mode for cellulosic ethanol production, and provid theoretical foundation and scientific basis for efficient production of cellulosic ethanol. The main results are as follows: We took advantage of bean hull, bean dreg, rice straw, beer lee and distillers’ grains as raw material for solid state fermentation. Firstly, the cellulase activity were compared among them when only one material as sole carbon source. And then choose the better of then, mixed and inoculated Neurospora crassa for solid state fermentation. The result show that the highest cellulose enzyme activity appeared in the bean hull、distillers’ grains and bean dreg mixed medium. The effects of medium composition were evaluated by using a Plackett-Burman design. The results showed that bean hull, distillers’ grains and (NH4)2SO4 were the main factors while the other additive had no significant effects on the cellulase production. Then, the central composite design and response surface analysis were used to determine the optimal levels of the main factors. The optimized conditions were：64% bean hull, 34% distillers’ grains and 2% ammonia sulfate. Under these conditions, the activity of cellulases significantly improved 141.88%, from 1.17IU/g to 2.83IU/g. The single-factor experiment had been used to