糖尿病与Th17细胞及其相关细胞因子关系的初步分析.docxVIP

青海大学硕士学位论文abstract 青海大学硕士学位论文 abstract III III Abstract Objective Type 1 diabetes is the autoimmune disease caused by environment and heredity. The pathogenesis of type1 diabetes is virus , diet and other external factors induce autoimmune response of islet , especially those who have the genetic susceptibility. T lymphocyte mediated autoimmune responses was activation cause immune damage of islet beta cells, beta cell mass and function declined, leading to elevated blood glucose. The Th17 cell is CD4+T cell subsets, which involved in the pathogenesis of many autoimmune diseases. In this study, the peripheral blood Th17 cells ratio and serum IL-17A, IL-23, IL-6,IL-1β levels were mesured in patients with type 1 diabetes, type 2 diabetes and healthy control group, comparison its expression difference among the three group. To understand the relationship between Th17 helper T cells and type 1 diabetes mellitus. Methods According to WHO diabetes diagnostic criteria of 1999,20 patients with type 1 diabetes mellitus (T1DM),21 patients with type 2 diabetes mellitus (T2DM) and 22 healthy controls were enrolled in the study.The course disease of T1DM about 6±0.3 months,an average age of T1DM with 17±11 years old;The course disease of T2DM about7±3.5 months，an average age of T2DM with 38±8 years old. The Th17、Tc17 cell in peripheral blood were detect by Flow cytometry;The levels of serum fasting plasma glucose (FPG) was measured by glucose oxidase method; the fasting C-peptide(FCP) and fasting insulin（FINS）were measured by magnetic separation enzyme- linked immunosorbent assay ; the levels of serum interleukin 17A (IL-17A)、IL-23、IL-6、IL-1β were measured by Enzyme-linked immunosorbent assay（ELISA）.White blood cells（WBC）、neutrophils（N）、 lymphocyte（L）、Alanine aminotransferase（ALT）、Aspartate transaminase（AST）、 blood albumin（ALB）、globulin（GLB）、hemoglobin（HGB）、blood urea（BUN） PAGE PAGE IV and serum creatinine（Cr）were detected by biochemical method;All of the statist