低强度超声对iPSCs源性神经嵴干细胞增殖和分化的影响-生物学专业论文.docx

重庆大学硕士学位 重庆大学硕士学位论文 英文摘要 PAGE PAGE VI ABSTRACT Injury of peripheral nerve is very common. Many kinds of trauma (such as compression, traction, tear and cut off) and other factors (such as ischemia and tumor) can cause partial or complete injury of nervous system, which will result in function loss of nerve and other neurological diseases. With the development of technology, the treatment of peripheral nerve injury has significantly been improved. However, the morpgologic and functional regeneration are still seldom achieved. Slow rates of nerve regeneration and functional recovery as well as the atrophy, degeneration and dysfunction of target organs are still the difficulty in clinics. Combined with the principle of peripheral nerve tissue engineering, induced pluripotent stem cells derived neural crest stem cells (iPSCs-NCSCs) were used as the source of seed cells in this study. Low intensity ultrasound stimulation (LIUS) was used to enhance the rate of peripheral nerve regeneration and functional recovery. In this thesis, the effects of LIUS on the viability, proliferation and differentiation (into neurons and Schwann cells (SCs)) of iPSCs-NCSCs cultured in induction media were emphatically investigated. The main works and results are as follows: ① Identify multi-lineages differentiation potential of iPSCs-NCSCs and the realization of LIUS at cellular level. Multilineage differentiation potential of iPSCs-NCSCs was investigated with osteogenic, chondrogenic, adipogenic and neural induced medium. The results showed that iPSCs-NCSCs had the ability to differentiate into osteoblast, chondrocytes, adipocyte, neurons and SCs with specific induced media. In this study, LIUS equipment (US10, purchased from Cosmogamma Corporation, Italy) was used to realize ultrasound stimulation with fixed frequency (1 MHz), duty cycle (20%), pulse repetition rate (100 Hz) and treatment time (10 min). Then the effects of LIUS on cell viability, proliferation and differentiation