单质纳米硒硫的可控制备、表征及细胞生物学性质-无机化学专业论文.docx

万方数据 万方数据 PREPARATION, CHARACTERIZATION AND CELL BIOLOGICAL PROPERTIES OF SELENIUM/SULPHUR NANOPARTICLES A Dissertation Submitted to the Graduate School of Henan University in Partial Fulfillment of the Requirements for the Degree of Master of Engineering By Xu Fenglan Supervisor: Dr Xing Ruimin Date: May, 2015 万方数据 万方数据 万方数据 万方数据 摘 要 纳米材料因在光催化、电化学、生物医学等领域都有着广泛的应用前景，成为当今 社会人们研究探索的热点问题。纳米硒因具有较高的生物活性、抗氧化性和低毒性被应 用于机体新陈代谢和生物医学等领域，并取得了很好的临床结果；硫则是在光催化方面 有很大的优势。硫元素因与硒元素同主族，也应该存在着很好的生物活性。 论文应用水热法与歧化法分别合成单质纳米 Se、S。利用 X 射线衍射和元素能谱对 产物的组成进行分析。利用扫描电镜和透射电镜对样品的形貌进行研究。利用热重与红 外光谱等手段对其稳定性能进行分析。通过研究单质纳米 Se、S 在体外对细胞活性的影 响，推测可能的反应机理。此论文主要研究成果总结如下： （1）纳米 Se 的制备和抗肿瘤活性研究：采用水热法制备纳米 Se，以牛血清白蛋白 做表面活性剂，通过改变反应时间来调控纳米 Se 颗粒的粒径大小。取纳米硒在体外作 用于乳腺癌与肝癌细胞，结果显示，纳米 Se 对肝癌细胞的抑制作用随着浓度的增加和 时间延长而增强，对乳腺癌细胞影响不大。 （2）单质 S 的制备和抗肿瘤活性研究：在酸性条件下，以硫代硫酸钠为硫源，利 用表面活性剂的添加与否控制合成单质 S。由 X 射线衍射图中衍射峰的变化，可得知两 组样品为两种形态，即结晶状态与无定形状态。取无定形单质硫在体外作用于肝癌细胞 与正常细胞，结果显示，单质硫对肝癌细胞的抑制作用随着浓度的增加和时间延长而增 强，对正常细胞影响不明显。 关键词： 纳米材料，纳米硒，纳米硫，细胞毒活性 I 万方数据 万方数据 ABSTRACT There was an increasing interest in nanomaterials, because of their wide application prospect in fields of photocatalysis, electrochemistry, biomedicine and so on. Nowadays, nano-selenium with higher bioactivity, oxidation resistance and hypotoxicity has been used in fields of the body’s metabolism and biomedicine, which had achieved good results.Sulfur has great advantage in the field of photocatalysis. Sulfur and selenium belong to the same group, so sulfur might have good bioactivity. In this paper, hydrothermal process and ion exchange method have been used to synthesize nano-selenium and nano-sulfur, respectively. The composition and morphology of the samples have been characterized by XRD, EDS, SEM and TEM. The stability of the samples has been characterized by TG-DTA and FT-IR. We have speculated the possible mechanism through the study of the effect of the nano-selenium and nano-sulfur on the bioactivity of the cancer cells in vitro. Hydrothermal process was applied to synthesize