低蛋白酶A酵母菌株的选育-微生物学专业论文.docx

Abstract Abstract Abstract Beer foam is an important indicator of beer quality. With 由e rise of draft beer in both intematíonal and domestic market， more 翩翩ion is focused on the foam stability of dra白 beer. The proteinase A excretes by yeast is rωognized 邸 the most important negative factor which influence foam stability. In this s阳dy，ultraviolet mutagenesis ，ion拍am mutation was adopted for breeding yeast w灿 low proteinase A excretion to solve the foam s阳bility of draft beer fundamen时ly. Five screening methods were compared in this study，including acid denatured bovine hemoglobin plate，CPY plate，96-well plate，Bradford method and fluorescent substrate. 96-well plates and Bradford method were used as preliminary and second screening method， respectively，fluorescent substrate is introduced ωdetermine the final content of pro阳inaseA. Ultraviolet mu阳genesis is adopted commonly in mutagenesis. The original brewing yeast M I 4 was induced by ultraviolet and four brewing yeωts with lower-level protein剧eA were bred. Compared with the original strain， the proteinase A activity of four mutation strains decreased in different rate，in which three mutants were reduced with lager extent. W-ion仇am mutation is a new method in recent years.ηle entire process experience physical ，chemical and biological four stages，which is a composite mutagenesis，with advantages as high efficiency，little injury and so on.η山study is the first report using 由is method on beer yeas. Mutating by this mean，two mutation strains were got 仕om the original yeast M 14. Compared with original strain，the proteinase A activity of the two mutation strains decreased different rate，but the reduction is not significantly ωtheUVmu阳genesis. Fermentation experiment found 由at fermentation performance of mutation strains reduced with distinct range. The results of EBCωbe showed that comparing with erlemeyer nωk fermentation，由e fermentation capability of mutation strains improved with different rate，bu