葛根素与松针挥发油配伍对大鼠离体胸主动脉环作用及机制的分析-中西医结合基础专业论文.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 PAGE PAGE 5 constraction of the vascular rings induced by KCl in a concentration-dependent positive correlation, which was not related to the function of endothelium at all. 3、Contraction elicited by 10-6mol/L NE via intra-cellular Ca2+ release was not changed significantly for the use of 10-5mo/L Puerarin with which the endothelium- denuded rings were incubated in calcium-free K-H fluid, but was changed via Ca2+ influx significantly while incubation with 2% Pine oil could inhibit the contraction via intra-cellular Ca2+ release and Ca2+ influx significantly. 4、Puerarin showed lower vasodilating function to the epithieum-intact ring pre-constricted by NE compared to control group after incubation with TEA and Gli while incubation with 4-AP showed no significant difference to the control group. However, 2% Pine oil showed no significant difference to control group pre- constricted with NE after incubation with TEA, 4-AP and Gli. 5、10-4mol/L Puerarin and 4% Pine oil 、10-5mol/L Puerarin and 4% Pine oil 、 10-6mol/L Puerarin and 4% Pine oil ,10-6mol/L Puerarin and 2% Pine oil showed best effects to inhibit the contraction of the vascular rings induced by NE. Vasodilatation of the combination group was significantly different from that of the singles and the combination is of synergic effect. Conclusions 1 、 The results indicate that the effects elicited by puerarin are endothelium-dependent and concentration-dependent, the vasodilation effect of puerarin may be mediated by inhbiting receptor-depent Ca2+ channel to decrease Ca2+ influx to vascular smooth muscle cells, its effects had no business with voltage-dependent Ca2+ channel, its effects were also partly mediated by the opening of Calcium-activated potassium channels and ATP-sensitive potassium channels. 2、The pine oil may act on the vascular smooth muscle cells directly and its vasodilation effects are concentration-dependent by inhibiting Ca2+influx and intra-cellular Ca2+re