弓形虫Prugniaud株感染诱导小鼠抑制黑色素瘤效应的实验研究-临床检验诊断学专业论文.docx

PAGE PAGE 1 分类号 R446.69 密 级 学校代码 1 0 3 6 7 U D C 577 编 号 学 号 20066831055 学 位 论 文 弓形虫 Prugniaud 株感染诱导小鼠黑色素瘤抑制作 用的实验研究 Experimental study on the effect of Toxoplasma gondii Prugniaud strain infection-induced resistance to mice malignant melanoma 程启媛 指导教师姓名 孙新 教授 蚌埠医学院病原生物学教研室 申请学位级别 硕 士 学 科 专 业 临床检验诊断学 论文提交日期 2009 年 4 月 论文答辩时间 2009 年 5 月 学位授予单位 蚌埠医学院 学位授予日期 2009 年 6 月 答辩委员会主席： 论 文 评 阅 人： 2009 年 4 月 目 录 中 文 摘 要·····················································································2 英 文 摘 要·····················································································4 前 言 ·························································································7 材 料 和 仪 器 ·············································································· 10 实 验 方 法··················································································· 12 实 验 结 果··················································································· 22 讨 论 ······················································································· 32 结 论 ······················································································· 35 参 考 文 献··················································································· 36 致 谢 ······················································································· 39 附录 A 中英文术语及缩略词对照表 ····················································· 40 附录 B 常用试剂配制 ······································································· 42 附录 C 个人简历及发表论文 ······························································· 44 附录 D 综述 ··················································································· 45 弓形虫 Prugniaud 株感染诱导小鼠抑制 黑色素瘤效应的实验研究 摘 要 目的：检测弓形虫（Toxoplasma gondii, TG）Prugniaud 株单独或联合卡介苗 （BCG）作用后对小鼠恶性黑色素瘤的抑制作用效果，并尝试从不同侧面探讨 其作用机制，为筛选可能具有诱发非特异性免疫保护作用的组分提供新的线索。 方法：将绿色荧光标记的转基因小鼠 GFP C57/B6 接种 B16 细胞，建立荷瘤模 型，将荷瘤小鼠随机分为 4 组，每组 9 只；两天后，TG/BCG/B16 组腹腔接种 弓形虫 Prugniaud 株（TG）和卡介苗（BCG），TG/B16 组接种 Prugniaud 株，