不同浓度胰岛素水平对高脂饮食大鼠主动脉的影响与机制分析.pdfVIP

The effects and mechanism of the different levels of insulin on the aorta in rats under high fat diet Abstract Objective: To inspect the change of intima-media thickness of aorta under high fat diet rats with hyperinsulinemia, and to investigate it ’s mechanisms by setting different insulin levels in Sprague Dawley rats fed with high fat diet. Methods: A total 32 male SD rats, health, aged 8 weeks, weight 180-200g, were randomly divided into four groups: the ND group was fed with standard diet; the HFD group was fed with high fat diet; the HFD+INS group was fed with high fat diet and treated by insulin (INS); the HFD+STZ group was fed with high fat diet and treated by streptozotocin (STZ). After 1 week of acclimation period and abrosia for 12 hours, the HFD+STZ group was induced with a single intraperitoneal injection of STZ (50mg/kg), other groups were injected buffer at the same dose instead. Three days later, the HFD+INS group was induced with subcutaneous injection of 1U/Kg long-acting insulin every morning, other groups were injected normal saline (NS) at the same dose instead. The changes of blood sugar and weight of rats were monitored. Used of color doppler ultrasound evaluation IMT of the aorta. The morphological changes of aorta tissues were observed through light microscopy after HE staining. the levels of body weight, FPG, TG, LDL-C, TC, HDL-C, FINS, HbA1c% were measured after 4 months. The HOMA-IR was evaluated. The expression of VEGF, TNF-ɑ, PGC-1ɑ, ApoE were analyzed by western blot. R