大蒜素对二甲基甲酰胺致小鼠急性肝损伤的预防作用-中国工业医学杂志.PDF

·144 · 　2008 6 2 1 3 　　ChineseJIndMed　June2008, Vo.l2 1 No.3　　 ＊ 邢国振, 贾凤兰, 阮明, 张宝旭 (, 　100083 ) 　　:　 (N, N-dmiehylformamid,eDMF)。 　 DMF, 48 h, (ALT)、 (AST) (LDH);, , HE, ; , (GSH)、 (GSSG) (MDA)。 　 , ALT、 AST LDH, ; ALT、 AST LDH, , GSH/GSSG, MDA。　 DMF。 :;; :R994.3　　:A　　:1002-221X(2008)03-0144-04 ProeciveeffecofallicinonacueliverinjuryinducedbyN, N-dimehylformamide ＊ XINGGuo-zhen, JIAFeng-lan, RUANMing, ZHANGBao-xu (DeparmenofToxicology, SchoolofPubliecHealh, BeijingUniversiy, Beijing100083, China) Absrac:ObjeciveTosudyhepreveniveeffecofallicinonacueliverinjuryinducedbyN, N-dimehylformamide (DMF)inmice.MehodsAnexpermienalhepaooxicinjurymodelwasmadebyinjecionofDMFoinvesigaeheprevenive effecofallicin.Thedosagesofallicinusedinhisexpermienwere7.5, 15, 30 mg/kg, for5 day,s heDMF(2.8 g/kg) wasgiven30 minlaeraferallicinadminisraionahe4 hday, 4 8 hlaeraferinjecionofDMF, heserumwereakenforhe measuremenofheaciviiesofalanineaminoransferase(ALT), asparaeaminoransferase(AST)andlacaedehydrogenase (LDH), andheliverissueswereakenforhisopahologicalexaminaion(HEsaining)bylighmicroscope.Theraioof GSH/GSSGandheconenofmalondialdehyde(MDA)inliverhomogenaeswerealsodeecedahesameime.ResulsThe resulsshowedhaadminisraionofDMF, heserumaciviiesofALT, ASTandLDHweresignificanlyincreased, andhede- generaionandnecrosiscouldbeseeninliverissue.Whileinallicinprereasedgroup, heserumaciviiesofheseenzymes weredominanlylowerhanhaofDMFgroup, hehisopahologicalchangesinliverwerealsosignificanlyamelioraedaferpre- reamenofallicin.Addiionally, heraioofGSH/GSSGinliverhomogenaewasalsoincreased, heconensofMDA, how- eve,rwasmarkedlyreducedaswellinallallicinprereaed.ConclusionAllicinh