柠檬醛对指状青霉菌丝体膜脂过氧化的影响-食品科学.PDF

32 2016, Vol.37, No.23 食品科学 ※基础研究 柠檬醛对指状青霉菌丝体膜脂过氧化的影响 欧阳秋丽，贾 雷，陶能国* （湘潭大学化工学院生物与食品工程系，湖南湘潭 411105 ） 摘  要：探讨柠檬醛对指状青霉菌丝体膜脂过氧化的影响。研究发现，经最小抑菌浓度（minimal inhibitory concentration MIC 2.0 μL/mL minimal fungicidal concentration MFC 4.0 μL/mL ， ， ）和最小杀菌浓度（ ， ， ）柠檬醛处 理后，指状青霉菌丝体发生扭曲变形且表面出现明显褶皱，表明细胞完整性遭到破坏。不同质量浓度柠檬醛处理指 ＋ 状青霉后，其胞外电导率及K 泄漏量均显著增加，而总脂质含量降低，说明柠檬醛处理能增加指状青霉细胞膜通 MIC MFC 透性，导致细胞膜结构遭受破坏。进一步研究发现， 、 柠檬醛处理促使指状青霉总丙二醛含量、过氧化氢 含量和脂氧合酶、过氧化物酶活性升高。本研究结果表明：柠檬醛处理可诱导指状青霉菌丝体发生严重的膜脂过氧 化损伤，损伤细胞膜结构，从而抑制指状青霉菌丝体生长。 关键词：柠檬醛；指状青霉；细胞膜；膜脂过氧化 Citral Inhibits Mycelial Growth of Penicillium digitatum Involving Membrane Peroxidation * OUYANG Qiuli, JIA Lei, TAO Nengguo (Department of Biotechnology and Food Engineering, College of Chemical Engineering, Xiangtan University, Xiangtan 411105, China) Abstract: The effects of citral on membrane lipid peroxidation of Penicillium digitatum were investigated in this study. After treated with citral at minimal inhibitory concentration (MIC, 2.0 μL/mL) and minimal fungicidal concentration (MFC, 4.0 μL/mL), the morphology of P. digitatum hyphae was evidently altered, as indicated by a wrinkled and rough surface, by causing loss of cytoplasm and distortion of mycelia. Extracellular conductivity and the leakage of potassium ions were significantly higher after citral treatment compared with the control, which indicated the increase of membrane permeability. Moreover, citral could decrea