大鼠全脑照射后早期表面遗传学变化特征的分析.pdfVIP

英文摘要 大鼠伞脑照射后早期表观遗传学变化特征的研究 Researchabout alterations of inthe rapid epigenetics _‘ P 一‘ ‘ _ - ‘ ‘ 一‘ ‘ 0tratalterWholebrainirradiation hippocampus Abstract To theroleof intheirradiation-induced investigate epigenetic Purpose memory the ofhistonH3 andDNA inthe deficiencybyobservingchanges acetylationmethylation ofratbrainradiationmodel． hippocampus injury Materialsandmethods 50-200 Youngadult，male rats(1 Sprague-Dawleyg， into 4-5 divided of contr01．Afterwholebrain weeks)were groups2Gy,30Gy,and total wasextractedfromirradiated andwholebrain30 irradiation，the protein hippocampus werecut thecenterofthe aLeica 埘[Ilcryosectionscoronallythrough impact using cryostat at7 and30 blotWas andthebrainsections days days．Westernperformed were immunohistochemieastained．ThealterationsofhistonH3 andDNMTsWas acetylation observedand analyzed． ResultsWeshowthatboth and30 4MVnominal leadto 2Gy Gy photonenergy deacreaseinhistonH3 inthe founda25％reduction significent acetyl