fabp7超表达与rnai真核达载体对人乳腺癌mcf7细胞增殖的影响.docxVIP

优秀毕业论文 精品参考文献资料 兰州大学硕士学位论文的增殖； 兰州大学硕士学位论文 的增殖；FABP7 cDNA的不同片段也可不同程度地促进MCF-7细胞的增殖。初 步探讨了FABP7基因对人乳腺癌MCF-7细胞增殖的影响。以上结果为进一步研 究FABP7基因的功能奠定了基础。 关键词：FABP7；载体构建；基因超表达；RNAi；乳腺癌细胞 H 兰州大学硕士学位论文The 兰州大学硕士学位论文 The effect of FABP7 overexpression and RNAi EU勋rvOt|C1一 expression vector on 13ronteratton of human breast cancer MCF．7 cells ABSTR ACT Objectives：Observe the effect of FABP7 overexpression and RNAi Eukaryotic expression vector on proliferation of human breast cancer MCF-7 cells．So as to further lay the foundation by researching functions of genes． Methods：FABP7 gene to the eDNA as a template．Acquiring the domain of C、 N and Link three parts of FABP7 gene through PCR．The digested double strands DNA were inserted into the downstream of promoter of linearized pcDNA3．1．After the identification of recombinant by PCR and double strands，the FABP7 eDNA、 domain fragments and FABP7-siRNA vector DNA were transfected into MCF-7．The proliferation of the cells Was determined by MTr method．The change of mRNA of the the FABP7 eDNA、Drawing cell count growth curve、domain fragments and FABP7·siRNA cells Was detected by RT-PCR assay,FCM detected the division of cell cycle． Results：PCR and double strands demonstrated that the domain fragments vector could be constructed．FABP7 eDNA be transfected into MCF-7 which induced MCF-7 cell to be restrained，howeverpcDNA3．1-N、pcDNA3．1一C、pcDNA3．1-Unk domain fragments and FABP7··siRNA were transfected into MCF--7 which induced MCF-7 cell to increase，among which pcDNA3．1-Link part increase obviously．There was significant(PO．05)．Agarose gel electrophoresis of RT-PCR showed domain fragments and FABP7一siRNA mRNA level increased and FABP7 eDNA mRNA level decreased evidently．FCM detected the division of cell cycle．The percentage of Gland G2 in pcDNA3．1-FABP7 and pcDNA3．1一N increased，the percentage of S decreased． 兰州大学硕士学位论文The 兰州大学硕士学位论文 The percentage of Gland G2 in peDNA3．1一C、pcDNA3．1-Unk and FABP7-siRNA decreased，the percentage of S increased． Conclusio