epsteinbarr病毒动态检测在鼻咽诊断和治疗中的意义.docxVIP

优秀毕业论文 精品参考文献资料 中南大学硕士学位论文 中南大学硕士学位论文 摘要 ％。在30例鼻咽癌患者治疗前和治疗后1、2、3、4、5、6、7周及3 月后复查9次EBV-VCA／IgA检测中，多数病例(17／24)治疗后 EBV-VCA／IgA降低，部分患者(6／24)治疗后EBV-VCA／IgA变化不 明显。 4．通过分析EBV-VCA／IgA、EBV-DNA与鼻咽癌肿瘤大小相关 性，发现EBV-DNA拷贝数与鼻咽癌肿瘤体积有一定相关性。 结论： 1．将巢式PCR技术与荧光定量PCR技术结合，成功建立了血浆 EBV-DNA的检测方法一一巢式荧光定量PCR。 2．通过动态监测鼻咽癌患者治疗过程中血浆EBV-DNA拷贝数， 明确了EBV-DNA水平与鼻咽癌治疗疗效、肿瘤大小的关系。 EBV-DNA在一定程度上反映鼻咽癌肿瘤大小。 3．EBV-VCA／IgA和EBV-DNA拷贝数联合用于鼻咽癌的早期筛 查，具有一定的临床价值。 关键词LMPl，EBV-DNA，EBV-VCA／IgA，EB病毒，鼻咽癌 n 中南大学硕士学位论文 中南大学硕士学位论文 ABSTRACT ABSTRACT Background and oh]ective： Epstein-Barr virus(EBV)is closely associated with nasopharyngeal carcinoma(NPC)，and EBV-VCA／IgA is served as a biomarker of NPC ill early stage．Recently,it was reported that EBV-DNA quantitive assay in plasma had some clinical significance to NPC early diagnosis．It has not been illuminated whether EBV．DNA was associated with NPC tumor size． In this present，the dynamic study of EBV-VCA／IgA and EBV-DNA Was conducted in treatment progress，and the association of EBV-DNA and NPC tumor size was investigated． Methods： 1．T0 definite the NPC patients with EBV．nle suspected NPC outpatients in ENT of Xiangya Hospital were pathologically diagnosed and EBV-LⅣm l was detected in biopsy tissues by immunohistochemistry from May 2006 to January 2008． 2．To determine patients and collect samples．NPC patients were as the objects if the NPC patients with positive EBV agreed to join in experiment group．The plasma and serum were collected at pre-treatment and olle，two，three，four,five，six，seven weeks and three months after treatment． 3．，nlc plasma EBV-DNA concentration Was analyzed by using fluorescent quantitative nested—PCR technique in Mx3000p．The median concentration Was calculated and dynamic pictures were drawn． 4．The levels of EBV-VCA／IgA were detected through ELISA assay and dynamic pictures were drawn by OD． 5．The NPC tumor size Was measured using magnetic resonance imagin