dna指纹技术对近交系小鼠产扩大群的遗传检测-基础兽医学专业毕业论文.docxVIP

甘肃农业大学博士论文 四代和第五代进行常规生化标记分析，发现被检的十三个生化基因位点(Car-2，Es．1，Es．3， Gpil，Hbb，Idh一1，Trf,Cc一2，Es．10，Mod·1，Pgm一1，Gpd．1 and Akp．1)中，三个近交品系小鼠 第四代的生化基因位点与国标完全一致。第五代的生化基因位点中，BALB／c小鼠的十 三个生化基因位点与标准一致；DB觥小鼠有一个位点即Idh．1出现异常；C57BL／6小 鼠的十个生化基因位点与标准一致，有三个位点即Idh．1、Es．3、Es．1出现异常。将 C57BL／6J、BAI，B／c和DBA／2近交系小鼠第四代和第五代的DNA指纹图与常规生化标 记分析法比较，发现DNA指纹技术显示异常的群体生化标记分析未见异常，生化标记 分析显示可疑或异常的群体内DNA指纹图差异明显，表明DNA指纹技术在近交系小 鼠扩大群遗传检测中比生化标记分析更加灵敏。 为了解近交系扩大群小鼠的各项血液指标变化情况，本研究对近交系扩大群小鼠的 第一代至第五代近交系小鼠进行了血液分析，发现第一代至第四代近交系扩大群小鼠血 液的各项指标均正常，第五代近交系扩大群小鼠虽然在遗传检测时发现了遗传突变或污 染，但各项血液指标均在正常值范围内。 上述结果表明，DNA指纹技术在近交系小鼠遗传检测中更具优势。本研究中所用 (GGAT)4寡核苷酸探针具有高度的多态性，利用此探针标记的DNA指纹技术可以对 C57BL／6J、BALB／c和DBA／2近交系扩大群小鼠进行遗传监测。 关键词：DNA指纹；近交系小鼠；生产扩大群；遗传监测 IV  甘肃农业大学博士论文 DNA fingerprinting in genetic monitoring of production ●d o ·‘ ● exoanslon Uolony ol-inbred strain mice Summary- The inbred strain mice which have genetic stability and phenotypic consistency are widely used for scientific studies．These mice are prone to genetic contamination or mutation during the reproduction．Experiment of using the contaminated animal stocks results in financial loss as well as wrong results．So it is very necessary to monitor the genetic quality of inbred strains．For a long time，the traditional methods in genetic monitoring of inbred strains include skin grifling test，coat color test and biochemical marker analysis，etc．DNA fingerprinting has been used for genetic monitoring of inbred strain mice with the development of molecular technology．The objective of this study is to evaluate DNA fingerprinting and the traditional biochemical marker method in genetic monitoring of the production expansion of inbred strain mice．Therefore，We can obtain a more exact，reliable and specific way of genetic monitoring for laboratory animals and build a basis for the standard and the molecular biology method which can be used in genetic monitoring of laboratory animals． DNA fingerprinting of BALB／c，C57BI_／6J and DBA／2 inbred strain species mice Was performed with the biotin—labele