巨噬细胞在结肠癌ct-26细胞5-fu耐门药中的作用及机制.docxVIP

第三军医大学硕士学位论文 缩略语表 英文缩写 英文全名 中文全名 TAMS tumor．associated macrophages 肿瘤相关巨噬细胞 CRC colorectal cancer 结肠癌 ATCC The American Type Culture collection 美国标准培养收集所 NM The normal medium 正常培养基 CM The macrophage conditioned medium 巨噬细胞培养上清 CM(5一FUl The 5．FU treated macrophage conditioned预先经5一FU处理的巨噬细 medium 胞培养上清 DNA deoxyribonucleic acid 脱氧核糖核酸 ml Milliliter 毫升 心 Ammonium persulfate 过硫酸铵 DMEM dulbeccots modified eagle medium 细胞培养基 PBS Phosphate Buffered Saline 磷酸盐缓冲液 BSA Bovine serllm albumin 牛血清蛋白 DMSO dimethyl sulfoxide 二甲基亚砜 g Gr锄 克 mg Milligram 毫克 mA Millianpere 毫安 PP percentage ofpositive cells 阳性细胞百分比 Rpm revolutions per minute 转每分 PVDF Polyvinylidene fluoride 聚偏二氟乙烯 CCK．8 Cell proliferation assay kit一8 细胞增殖检测试剂盒 oD Opltical density 光密度值 ddH20 double distilled H20 双蒸水 TEMED N，N，N’，N’．tetra methyl ethylene diamine 四甲基乙烯基二胺 SDS sodium dodecyl sulfate 十二烷基磺酸钠 SDS．PAGE sodium dodecyl sulfate．polyacrylamide 十二烷基磺酸钠一聚丙烯酰 gel electrophoresis 胺凝胶电泳 万方数据  第三军医大学硕士学位论文 SDS sodium dodecyl sulfate 十二烷基磺酸钠 SDS．PAGE sodium dodecyl sulfate—polyacrylamide 十二烷基磺酸钠．聚丙烯酰 gel electrophoresis 胺凝胶电泳 BCA BCA Protein Assay Kit BCA蛋白浓度测定试剂盒 SPSS statistical package for social science 社会科学统计软件包 2 万方数据  第三军医大学硕士学位论文 The role of macrophages in resistance to 5-FU in colorectal cancer cell CT．26 Abstract Colorectal cancer(CRC)is one of the most common malignant tumors in clinic，which cause serious damage to human health．At present，as a single or combination drug therapy， 5．FU iS still the first choice for clinical practice to treat colorectal cancer．Recently，it iS reported that more than a half of patients developed resistence to 5一FU，and the majority is due to the reduced susceptibility to 5-FU after sustmned using．It is well established that chemotherapy drugs Can play its anti-tumor effect via inducing tumor cell apoptosis．Hence， inhibiting the apoptosis of tumor cell induced by chemotherapy drugs will lead to the chemoresistance．Consistently．our recent study in vitro had found that 5-FU played