基于phb的纳米纤维材料的制备及ecm应用-生物化学与分子生物学专业论文.docxVIP

Abstract II Abstract Polyhydroxyalkanoates (PHA) have been demonstrated to be a family of biopolymers with good biodegradability and biocompatibility. To mimic the real microenvironment of extracellular matrix (ECM) for cell growth, novel nano-fibrous matrices based on PHA polymers were prepared via a solution freeze drying process. Three-dimensional interconnected fibrous networks were observed in these matrices with average fiber diamaters of 50 ~ 500 nm, which are very similar to the major ECM component collagen. Compared with a nano-fibrous polyhydroxybutyrate (PHB) matrix, the mechanical properities of blended PHA nano-fibrous matrices were significantly improved, especially those matrices of PHA blends PHB/PHBHHx containing polyhydroxybutyrate (PHB) and copolyesters PHBHHx consisting of 3-hydroxybutyrate and 3-hydroxyhexanoate, and PHB/P3HB4HB that are PHB blended with copolyesters of 3-hydroxybutyrate and 4-hydroxybutyrate, respectively. More important, cell attachment and growth of human keratinocyte cell line HaCat on the nano-fibrous PHA matrices showed a notably improvements over those on PHA films prepared via an ordinary solution casting method. It was therefore proposed that PHA nano-fibrous matrices combine the advantages of biodegradation, improved mechanical strengthes, the architecture of a natural extracellular matrix, leading to a better cell compatibility, they can be used for future implant biomaterial development. Keywords: PHB；Polyhydroxyalkanoates； Nanofiber； Matrix； 目 录  PAGE 4 目 录 摘 要 I  HYPERLINK \l _bookmark0 Abstract II  HYPERLINK \l _bookmark1 目 录 III  HYPERLINK \l _bookmark2 第 1 章 前言 1  HYPERLINK \l _bookmark2 1.1 聚羟基脂肪酸酯（PHA）简介 1  HYPERLINK \l _bookmark3 1.2 PHA的应用 2  HYPERLINK \l _bookmark3 1.2.1 PHA的生物学功能 2  HYPERLINK \l _bookmark4 1.2.2 PHA作为生物可降解材料的应用 4  HYPERLINK \l _bookmark5 1.2.3 PHA在医用材料方面的应用 5  HYPERLINK \l _bookmark5 1.2.3.1 组织工程的研究内容和现状 5  HYPERLINK \l _bookmark6 1.2.3.2 PHA作为组织工程材