植物miRNA在非生物逆境下调控机制的研究进展.PDFVIP

安徽农业大学学报, 2013, 40(4): 673-677 Journal of Anhui Agricultural University 网络出版时间：2013-7-12 10:25:56 [URL] /kcms/detail/34.1162.S1025.003.html 植物 miRNA 在非生物逆境下调控机制的研究进展 1 2 2 2* 张世华 ，上官明珠 ，盛 亮 ，韦朝领 (1. 安徽农业大学生命科学学院，合肥 230036 ；2. 安徽农业大学茶与食品科技学院，合肥 230036) 摘 要：MicroRNA(miRNA)是一类内源性的、长度约 18 ～24 nt 、非编码、单链小RNA 分子，miRNA 广泛存 在于各种动植物体内，并参与基因表达的调控。随着植物miRNA 研究的不断发展，越来越多的miRNA 被证实在 不同的非生物逆境下具有重要的调控作用，例如营养胁迫、低温胁迫、机械胁迫等。该文综述了植物miRNA 的合 成、miRNA 及其靶基因的鉴定方法，并重点介绍了非生物逆境相关miRNA 的表达分析方法、逆境条件下miRNA 的调控特征及其靶基因特点等。 关键词：植物；microRNA ；非生物逆境；调控机制 中图分类号：Q75 文献标识码：A 文章编号：1672−352X (2013)04−0673−05 Research progress of plant miRNA regulatory mechanisms under the condition of abiotic stresses 1 2 2 2 ZHANG Shi-hua , SHANGGUAN Ming-zhu , SHENG Liang , WEI Chao-ling (1. School of Life Sciences, Anhui Agricultural University, Hefei 230036; 2. School of Tea and Food Science, Anhui Agricultural University, Hefei 230036) Abstract: MicroRNAs (miRNAs) are a class of endogenous, small (~18-24 nt), single-stranded, non-coding RNAs that widely distribute in animals and plants and participate in the regulation of gene expression. With the development of the research of plant miRNA, more and more miRNAs was convinced to play important regula- tory roles in the condition of abiotic stresses, e.g., nutrition starvation, low temperature, mechanism damage and so on. This paper reviews the synthesis of miRNA, the identification of miRNA and the targeted gene, and intro- duce the express analysis of stress-related miRNA, the regulatory pattern of miRNA and the property of the tar- geted gene.