麻布七中高乌甲素的含量测定--毕业论文.docx

四川理工学院毕业论文 读万卷书 行万里路 PAGE 20 读万卷书 行万里路 四川理工学院本科毕业论文 麻布七中高乌甲素的含量测定 学 生： 学 号： 专 业：制药工程 班 级：制药2013卓越 指导教师：穆祯强 四川理工学院化学工程学院 二〇一七年五月 读万卷书 行万里路 读万卷书 行万里路 摘 要 麻布七系毛茛科乌头属植物高乌头（Aconitum sinomontanum Nakai）的根，产自河北、甘肃和四川等地。其性温、味苦、有毒，具有祛风除湿，活血止痛的功效，用于风湿腰腿痛、胃气痛、跌打损伤的治疗。本文通过水提醇沉法和pH梯度萃取法得到麻布七生物碱部位，然后应用高效液相色谱，采用标准曲线法测定了生物碱部位中高乌甲素含量为36.54%，由此计算高乌甲素在麻布七中的含量为0.68%，从而为麻布七和含有麻布七药材的成药药品质量控制提供了参考，为麻布七在现有临床应用基础上的开发利用奠定了基础。 关键词：麻布七；高乌甲素；高效液相色谱；含量测定 ABSTRACT Root of Tall Monkshood derived from Aconitum sinomontanum Nakai of root, a species of the Ranunculaceae in Aconitum Medical herbs. growing?in?Hebei, Gansu, Si chuan and?other?places. Its temperature, bitter, toxic, with dampness, relieving pain ect.?It?is?used?for?the?treatment of?rheumatoid?low?back?pain, Stomach, bruises. In this paper, the alkaloid parts of Root of Tall Monkshood were obtained by water extraction and pH gradient extraction, and then using HPLC, using the standard curve method to determine the alkaloid site in the high eugenol content of 36.54%. The calculated content of Lappaconitine in Root of Tall Monkshood was 0.68%. So as to provide a reference for the quality control of medicine and medicine containing Root of Tall Monkshood medicine, which laid the foundation for the development and utilization of Root of Tall Monkshood on the basis of existing clinical application. Key?words: Root of Tall Monkshood; Lappaconitine; HPLC; Determination content 读万卷书 行万里路 读万卷书 行万里路 目录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc483940599 摘 要 PAGEREF _Toc483940599 \h I HYPERLINK \l _Toc483940600 ABSTRACT PAGEREF _Toc483940600 \h II HYPERLINK \l _Toc483940601 第一章 绪论 PAGEREF _Toc483940601 \h 1 HYPERLINK \l _Toc483940602 1.1 前言 PAGEREF _Toc483940602 \h 1 HYPERLINK \l _Toc483940603 1.2 麻布七的化学成分 PAGEREF _Toc483940603 \h 1 HYPERLINK \l _Toc483940604 1.3 高乌甲素的药理作用 PAGEREF _Toc483940604 \h 2 HYPERLINK \l _Toc483940605 1.3.1 镇痛作用 PAGEREF _Toc483940605 \h 2 HYPERLINK \l _Toc483940606 1.3.2 局部麻醉作用 PAGEREF _T