拮抗细菌枯草芽孢杆菌T429喷雾干燥工艺研究-生物学杂志.PDF

30(1)101-106 中国生物防治学报 Chinese Journal of Biological Control 2014 年 2 月 拮抗细菌枯草芽孢杆菌T429喷雾干燥工艺研究 孟祥坤，于俊杰，尹小乐，聂亚锋，俞咪娜，陈志谊，刘永锋* （江苏省农业科学院植物保护研究所，南京 210014 ） 摘要：本研究前期成功分离获得1 株对水稻真菌病害具有较强抑制作用的枯草芽孢杆菌T429 ，为开发生防 菌农药新剂型，利用喷雾干燥法对生防菌 T429 发酵液进行处理，以喷雾干燥后产物活菌量为主要指标， 采用单因素和正交试验法，对影响喷雾干燥产物指标的主要因素进行优化。结果表明，最佳喷雾干燥条件 为进风温度120 ℃、进样速度720 mL/h、雾化口径1 mm、雾化压力0.1 MPa、热风流量30 M3/h ，可获得 9 产物活菌量达8.61 ×10 cfu/g 。该工艺稳定、可控，为以枯草芽孢杆菌为核心的微生物杀菌剂新剂型的研 发提供了科学依据。 关 键 词：枯草芽孢杆菌；农药剂型；喷雾干燥；正交试验 中图分类号：S476 文献标识码：A 文章编号：1005-9261 (2014)01-0101-06 Study on the Spray Drying Technology of Antagonistic Bacteria Bacillus subtilis T429 * MENG Xiangkun, YU Junjie, YIN Xiaole, NIE Yafeng, YU Mina, CHEN Zhiyi, LIU Yongfeng (Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China) Abstract: Bacillus subtilis T429 was confirmed to be highly bacteriostatic to rice fungus diseases in previous study. Fermentation broth of B. subtilis T429 was granulated by spray-drying technology to develop new bacterial pesticide formulation. Single factor test and experiment with four-factor and three-level orthogonal were carried out to optimize spray-drying condition. Results showed that the optimum conditions were inlet air temperature 120 ℃ , the feed flow rate 720 mL/h, atomization caliber 1 mm, atomization pressure 0.1 MPa, hot air flow 30 3 9 M /h. The number of viable cells of products reached to 8.61 ×10 cfu/g under the optimal conditions. These data helps development of new pesticide formulation of B. subtilis . Key words: Bacillus subtilis ; pesticide formulation; spray drying; orthogonal experiment 枯草芽孢杆菌 Bacillus subtilis 属于革兰氏阳性细菌，广泛分布于土壤、湖泊、动植物体表等处[1]