延龄草根及根茎的化学成分研究II.PDF

·2808 · 中草药 Chinese Traditional and Herbal Drugs 第44 卷 第20 期 2013 年10 月 延龄草根及根茎的化学成分研究 （II ） * 张忠立，左月明 ，蔡妙婷，王彦彦 江西中医药大学药学院，江西 南昌 330006 摘 要：目的 研究延龄草Trillium tschonoskii 根及根茎中的化学成分。方法 采用硅胶、聚酰胺、反相硅胶C18 和Sephadex LH-20 柱色谱对延龄草70%乙醇提取物进行分离纯化。用质谱、核磁等波谱学方法确定化合物结构。结果 从延龄草醋酸乙 酯和正丁醇部位分离得到 12 个化合物，分别鉴定为 β-蜕皮激素（1）、pinnatasterone （2 ）、polypodine B （3 ）、3- 甲氧基-4- 羟基-苯丙酸甲酯（4 ）、岷江百合苷 A （5 ）、4-羟基苯甲酸（6 ）、香草醛（7 ）、β-D-吡喃葡萄糖基(1→4)-O-[α-L-吡喃鼠李糖 基 (1→2)]-O-β-D-吡喃葡萄糖苷（8 ）、重楼皂苷V （9 ）、重楼皂苷III （10）、延龄草苷A （11）、延龄草苷C （12）。结论 化 合物1、2 、6、7、9、10 首次从该属植物中分离得到，化合物3～5、11、12 首次从该种植物中分离得到。 关键词：延龄草；岷江百合苷A ；重楼皂苷V ；延龄草苷A ；β-脱皮激素 中图分类号：R284.1 文献标志码：A 文章编号：0253 - 2670(2013)20 - 2808 - 04 DOI: 10.7501/j.issn.0253-2670.2013.20.004 Studies on chemical constituents in roots and rhizomes of Trillium tschonoskii (II) ZHANG Zhong-li, ZUO Yue-ming, CAI Miao-ting, WANG Yan-yan College of Pharmaceutical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China Abstract: Objective To investigate the chemical constituents in the roots and rhizomes of Trillium tschonoskii. Methods The roots and rhizomes of T. tschonoskii were extracted with 70% ethanol and separated by chromatography on polyamide, silica gel, RP-C18, and Sephadex LH-20 columns. Chemical structures were identified by MS, 1D and 2D NMR experiments. Results Twelve compounds were isolated and identified from the ethyl acetate extract from the roots and rhizomes of T. tschonoskii and n-butanol fractions were identified as β-ecdysone (1), pinnatasterone (2), polypodine B (3), methyl ferulorate (4), regaloside A (5), 4-hydroxy- benzoic acid (6), vanillic aldehyde (7), β-D-glucopyranosyl-(1→4)-O-[α-L-rhamnopyranosyl-(1→2)]-O-β-D-glucopyranoside (8), paris saponin V (9), paris saponin III (10), trillenoside A (11), and trillenoside C (12). Concl