基于G-四链体构建DNA比色逻辑门的研究-分析化学专业论文.docxVIP

Abstract that single-stranded (ss) DNA can be released. The released ssDNA can hybridize to signal reproter DNA (two G-rich DNA strands), self-assembling into the hemin/G-quadruplex that biocatalyzes the oxidation of TMB by H2O2, resulting in a colorimetric signal. Based on this principle, we constructed a series of logic gates (OR, AND, INHIBIT, XOR) by using Hg2+ and Ag+ ions as inputs and the colorimetric signal as output. In the other case, the released ssDNA can hybridize to the other signal reproter DNA (self-assembled split G-quadruplex structure), leading to the disassembly of the split G-quadruplex, resulting that hemin/G-quadruplex can not be formed and the colorimetric signal can not be produced. Based on this mechnism, we employed Hg2+ and Ag+ ions as inputs and colorimetric signal as output to construct NOR, NAND, IMPLICATION and XNOR logic gates. Besides, this method also implements the logic detection for heavy metal ions (Hg2+ and Ag+) in the OR logic gate, demonstrating that it has a good application prospect. Key Words: Colorimetric Logic Gates; Triplex-Helix Molecular Switch; G-quadruplex; DNAzyme; Exo III; Metal ions 目 录 HYPERLINK \l _bookmark0 第一章 绪论 1 HYPERLINK \l _bookmark1 1.1 引言 1 HYPERLINK \l _bookmark2 1.2 DNA 逻辑门的概述 2 HYPERLINK \l _bookmark3 1.2.1 DNA 逻辑门的概念 2 HYPERLINK \l _bookmark4 1.2.2 DNA 逻辑门的基本原理 2 HYPERLINK \l _bookmark5 1.3 DNA 逻辑器件的组成以及分子识别方法 3 HYPERLINK \l _bookmark6 1.4 DNA 逻辑门的构建方法 5 HYPERLINK \l _bookmark7 1.4.1 基于 DNA 构型变化构建 DNA 逻辑门 5 HYPERLINK \l _bookmark8 1.4.2 基于调控酶活性构建 DNA 逻辑门 6 HYPERLINK \l _bookmark9 1.5 DNA 比色逻辑门的构建 8 HYPERLINK \l _bookmark10 1.6 本论文主要研究内容 10 HYPERLINK \l _bookmark11 第二章 基于三链 DNA 构型变换构建比色逻辑门11 HYPERLINK \l _bookmark12 2.1 引言 11 HYPERLINK \l _bookmark13 2.2 实验部分 12 HYPERLINK \l _bookmark14 2.2.1 试剂与仪器 12 HYPERLINK \l _bookmark15 2.2.2 实验方法 13 HYPERLINK \l _bookmark16 2.3 结果与讨论 14 HYPERLINK \l _bookmark17 2.3.1 功能分子三链发夹信标分子（tMB）的设计原理 14 HYPERLINK \l _book