Acinetobacter sp TW生物强化反应器抗尼古丁负荷冲击能力分析.docVIP

Acinetobacter sp. TW生物强化反应器抗尼古丁负荷冲击能力分析 摘要：为评估高效尼古丁降解菌 Acinetobacter sp. TW 生物强化反应器的工程应用潜能，本实验考察了在尼古丁负荷冲击下，系统对目标污染物的去除效能，并从群体生态学和抗氧化应激角度对其机理进行了分析。结果表明，当反应器受到 2 倍(500 mg/L)尼古丁负荷冲击时，运行效能不受影响；当分别受到 3 倍(750 mg/L)、4 倍(1 000 mg/L)、5 倍(1 250 mg/L)和 6 倍(1 500 mg/L)冲击时，短时间内反应器的运行效能与负荷冲击浓度呈反相关，但仍能在冲击后24-72h内恢复至原水平。微生物群体生态学指标显示，不同尼古丁浓度冲击前后微生物群落结构差异不大，但冲击过程中长、短链信号分子分别由基准值 54.4 ± 0.1 Millier unit和 72.3 ± 0.1 unit上升至 82.2 Millier unit 和 84.1 unit，冲击后 72 h 内回落至正常值，表明群体感应可在微生物抵抗负荷冲击过程中发挥作用。抗氧化应激指标显示，随着负荷冲击浓度的增加，ROS和蛋白羰基含量分别由基准值 205 ± 1 U/mL 和 1.06 ± 0.1 nmol/mg 梯度上升至 452.2 U/mL和 1.68 nmol/mg，与尼古丁负荷冲击浓度呈明显正相关，但均可在 72 h 内回落至正常值，表现出良好的抗冲击性能。 关键词：生物强化；负荷冲击；种群结构；群体感应；氧自由基  The resistant potential against nicotine shock of Acinetobacter sp. TW bioaugmented system Abstract：With the attempt to evaluate the potential of engineering application of Acinetobacter sp. TW bioaugmented system, the performance of the bioaugmented system under different nicotine shock was measured. Meanwhile, the mechanism related to it was discussed according to the theories of quorum sensing and oxygen free radicals. The result showed that when the reactor received 2 times (the concentration of nicotine in inflow was 500 mg/L) nicotine shock it almost had no effect on the performance of the reactor. When the reactor received 3 times (750 mg/L), 4 times (1 000 mg/L), 5 times (1 250 mg/L) and 6 times (1 500 mg/L) nicotine shock, the performance of the reactors went worse as the concentration of nicotine ascended. However, it only took 24-72h for the reactors to perform well again. The result related to microbial community indicated that the concentration of both long-chain AHLs and short-chain AHLs went up from 54.4 ± 0.1 Millier unit and 72.3 ± 0.1 unit to 82.2 Millier unit and 84.1 unit, respectively. And it only took 72 h for the reactors to perform well again, which indicated that quorum sensing took part in the behavior of nicotine shock resistance. The result related to ecotoxicology indicated that as the c