甜酒曲中产糖化酶根霉菌株紫外诱变选育-食品科学.PDFVIP

204 2012, Vol. 33, No. 07 食品科学 ※生物工程 邓开野，林佩芳，黎尔纳 (仲恺农业工程学院轻工食品学院，广东　广州 510225) 从甜酒曲中分离出产糖化酶根霉菌株，通过平板菌落初筛、测定HE 值和摇瓶复筛测糖化力，筛选出 1 株HE 值大且糖化力高的优良出发菌株，对其进行紫外线诱变处理，选育得到优良变异株，该菌株的 HE 值为 1.76，糖化 力为 1281mg/(h ·g) ，分别较出发菌株高出5.4%、59.5% 。对此优良变异株的培养条件进行优化，获得最佳的培养条 件为：培养温度为 30 ℃，培养时间为3d ，添加玉米粉质量浓度为8g/100mL，在此条件下糖化力为 1317mg/(h ·g) 。 甜酒曲；根霉；紫外线诱变；糖化力 Breeding of a Glucoamylase-Producing Strain of Rhizopus from Sweet Rice Wine Starter by Ultraviolet Radiation DENG Kai-ye ，LIN Pei-fang ，LI Er-na (College of Light Industry and Food Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China) Abstract ：An excellent strain of Rhizopus with high HE value and saccharification power from sweet rice starter was screened through flat plate primary screening and mutagenized by UV radiation. An excellent mutant strain with an HE value of 1.76 and a saccharification power of 1281 mg/(h ·g) was obtained, which were increased by 5.4% and 59.5% compared to those of the original strain, respectively. The optimal culture conditions for the mutant strain were culture temperature of 30 ℃, culture time of 3 days and corn flour concentration of 8 g/100 mL. Under the optimal conditions, the saccharification power of the screened strain was up to 1317 mg/(h ·g). Key words ：sweet rice starter ；Rhizopus ；UV-induced mutagenesis ；saccharification power 中图分类号：Q933 文献标识码：A 文章编号：1002-6630(2012)07-0204-05 肪酶[8] [9] 中国酒曲历史悠久，有的学者认为，制曲酿酒可 、酯化酶 等研究较多，而对酒曲中产糖化酶的 与我国古代四大发明相媲美[ 1 -2 ] 。在我国南方各省，小 根霉研究较少，对甜酒曲中产糖化酶根霉菌株的诱变选 曲酒在市场中都占据一定优势，其价廉物美，深受广 育则未见报道。为了提高根霉菌株的产酶能力，实验从 大消费者的欢迎[ 3 ] 。小曲酒生产用曲量小，生产周期