高脂对小鼠Foxp3基因表达的影响.docVIP

PAGE PAGE 4 高脂对小鼠Foxp3基因表达的影响 王治校1,2，王崇全2，何朝荣2，詹中群2，王俊峰2，李大主1 （430021 武汉，华中科技大学附属协和医院心血管内科1；442000湖北 十堰，郧阳医学院附属太和医院心血管内科2） [摘要] 目的 研究高脂对小鼠Foxp3基因表达的影响。方法 实验动物分为对照组和高脂饮食组。构建小鼠动脉粥样硬化模型，比较不同组小鼠血脂水平、斑块面积和不同器官组织中Foxp3+CD4+CD25+Tregs细胞的数目；利用RT-PCR和Western blot分析组织中Foxp3基因转录水平和Foxp3蛋白表达水平。结果 与对照组比较，高脂饮食组小鼠血脂水平[TG(2.8±0.19)mmol/L、TC(28.2±1.2) mmol/L]明显升高，斑块面积(25 044.32±4 920.42)μm2显著增加,而不同器官组织中Foxp3+CD4+CD25+Tregs细胞的数目显著减少(P0.05)，Foxp3基因转录水平(P0.001)和Foxp3蛋白表达(P0.05)降低。结论 高脂能抑制不同免疫器官Foxp3基因的表达和功能，可能是高脂致动脉粥样硬化的机制之一。 [关键词] 高血脂；Foxp3基因；动脉粥样硬化；Foxp3+CD4+CD25+ Tregs细胞 [中图法分类号] R541.4 [文献标志码] A Effects of hyperlipidemia on expression of FOXP3 in WANG Zhi-Xiao1, 2, WANG Chong-Quan2, HE Chao-Rong2, ZHAN Zhong-Qun2, WANG Jun-Feng2, Li Da-Zhu1(Department of Cardiology, Taihe hospital, YunYang Medical College, Shiyan Hubei 442000; Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science Technology, Wuhan 430022) [Abstract] Objective The aim of this study was to examine the effect of hyperlipidemia on expression of Foxp3 gene. Methods At 8 weeks of age, 16 male ApoE?/? mice were randomly divided into control group and high fat diet group of eight. To compare the level of blood fat in C57BL/6 mice through constructing the model of atherosclerotic plaque in ApoE?/? mice. The resulting atherosclerotic lesions were assessed by determining Foxp3 gene transcript levels and investigating the expression of Foxp3 protein in different tissues. Results The level of blood fat (TG (2.8±0.19)mmol/L、TC (28.2±1.2)mmol/L)was increased significantly, at the same time, the number and function of Foxp3+CD4+CD25+Tregs(P0.05), Foxp3 protein levels(P0.05) and Foxp3 gene transcript levels(P0.001) in different tissues were low in the high fat diet group compared with the control group given normal food. The area of atherosclerotic lesions in the high fat diet group (25044.32 ± 4920.42) μm2 were higher than (597.38±305